Neutrophil collagenase in sputum from patients with cystic fibrosis

Power, C, O'Connor, C M, MacFarlane, D, O'Mahoney, S, Gaffney, K ORCID:, Hayes, J and FitzGerald, M X (1994) Neutrophil collagenase in sputum from patients with cystic fibrosis. American Journal of Respiratory and Critical Care Medicine, 150 (3). pp. 818-822. ISSN 1073-449X

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The potential role of neutrophil elastase in causing lung damage and exacerbating the inflammatory response in cystic fibrosis (CF) has received considerable attention. Although another potent neutrophil-derived enzyme, collagenase, is implicated in tissue destruction in several interstitial lung disorders, there has been no reference to this enzyme in CF. The objective of this study was to determine whether neutrophil collagenase is present in active form in CF sputum and, if so, whether it is related to disease severity. High levels of active collagenase were detected in sputum from patients with CF, and the majority of the enzyme present was of neutrophil origin. In a group of 16 patients with CF, negative relations between sputum collagenase activity and Shwachman score (r = -0.55, p < 0.05) and FEV1 (r = -0.59, p < 0.02) were noted, indicating an association between high collagenase activity and severity of disease. A positive correlation was observed between sputum collagenase and elastase activity (r = 0.62, p < 0.05). These results suggest that both neutrophil elastase and collagenase may play a significant role in lung destruction in CF.

Item Type: Article
Uncontrolled Keywords: adolescent,adult,analysis,enzymology,electrophoresis, polyacrylamide gel,female,humans,immunoblotting,analysis,male,molecular weight,enzymology,analysis,enzymology,regression analysis,severity of illness index,enzymology
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 26 Aug 2020 00:02
Last Modified: 22 Oct 2022 06:40
DOI: 10.1164/ajrccm.150.3.8087357

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