Synergism of sophoraflavanone G with norfloxacin against effluxing antibiotic-resistant Staphylococcus aureus

Sun, Zhong-Lin, Sun, Shi-Chang, He, Jian-Ming, Lan, Jiang-Er, Gibbons, Simon and Mu, Qing (2020) Synergism of sophoraflavanone G with norfloxacin against effluxing antibiotic-resistant Staphylococcus aureus. International Journal of Antimicrobial Agents. ISSN 0924-8579

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Abstract

Staphylococcus aureus including methicillin resistant variants (MRSA) is still a challeng in the clinical and community settings. The design and discovery of new compounds to deal with resistant bacteria has become one of the most important areas of antiinfective research today. The goal of this study was to address the problem of MRSA by searching for synergistic antibacterial natural products fromtraditional Chinese herbs that are not substrates for the efflux mechanisms of MRSA strains and that overcome drug-resistance of bacteria by other, as yet, undescribed mechanisms. In vitro, synergistic activity was determined using the standard checkerboard method, and mechanistic studies were performed by an ethidium bromide (EtBr) efflux assay. Using in vivo experiments, the efficacies of different concentrations of the combinations were compared in a murine model of pyaemia. The natural product sophoraflavanone G (SG) showed specific synergistic antibacterial effects both in vitro and in vivo and may serve as a template for agents with antibiotic-potentiating activity for use against infections caused by S. aureus.

Item Type: Article
Faculty \ School: Faculty of Science > School of Pharmacy
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 22 Jul 2020 23:41
Last Modified: 22 Jul 2020 23:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/76242
DOI: 10.1016/j.ijantimicag.2020.106098

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