Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus
Tools
ToolsGoodman, Timothy, Nayar, Stuart G., Clare, Shaun, Mikolajczak, Marta, Rice, Ritva, Mansour, Suzanne, Bellusci, Saverio and Hajihosseini, Mohammad K. (2020) Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus. Development, 147 (13). ISSN 0950-1991
|
PDF (Published manuscript)
- Published Version
Available under License Creative Commons Attribution. Download (13MB) | Preview |
Abstract
New neurons are generated in the postnatal rodent hypothalamus, with a subset of tanycytes in the third ventricular (3V) wall serving as neural stem/progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing β-tanycytes at postnatal days (P)4-5 results in the generation of significantly more parenchymal cells by P28, composed mostly of ventromedial and dorsomedial neurons and some glial cells, which persist into adulthood. A closer scrutiny in vivo and ex vivo revealed that the 3V wall is not static and is amenable to cell movements. Furthermore, normally β-tanycytes give rise to parenchymal cells via an intermediate population of α-tanycytes with transient amplifying cell characteristics. Loss of Fgf10 temporarily attenuates the amplification of β-tanycytes but also appears to delay the exit of their α-tanycyte descendants from the germinal 3V wall. Our findings suggest that transience of cells through the α-tanycyte domain is a key feature, and Fgf10 is a negative regulator of postnatal hypothalamic neurogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cell movement,fibroblast growth factors,hypothalamus,lineage tracing,mouse,postnatal neurogenesis,tanycytes,molecular biology,developmental biology ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 22 Jul 2020 02:46 |
| Last Modified: | 05 Feb 2021 02:42 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/76216 |
| DOI: | 10.1242/dev.180950 |
Actions (login required)
 |
View Item |