Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus

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Goodman, Timothy, Nayar, Stuart G., Clare, Shaun, Mikolajczak, Marta, Rice, Ritva, Mansour, Suzanne, Bellusci, Saverio and Hajihosseini, Mohammad K. (2020) Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus. Development, 147 (13). ISSN 0950-1991

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Abstract

New neurons are generated in the postnatal rodent hypothalamus, with a subset of tanycytes in the third ventricular (3V) wall serving as neural stem/progenitor cells. However, the precise stem cell niche organization, the intermediate steps and the endogenous regulators of postnatal hypothalamic neurogenesis remain elusive. Quantitative lineage-tracing in vivo revealed that conditional deletion of fibroblast growth factor 10 (Fgf10) from Fgf10-expressing β-tanycytes at postnatal days (P)4-5 results in the generation of significantly more parenchymal cells by P28, composed mostly of ventromedial and dorsomedial neurons and some glial cells, which persist into adulthood. A closer scrutiny in vivo and ex vivo revealed that the 3V wall is not static and is amenable to cell movements. Furthermore, normally β-tanycytes give rise to parenchymal cells via an intermediate population of α-tanycytes with transient amplifying cell characteristics. Loss of Fgf10 temporarily attenuates the amplification of β-tanycytes but also appears to delay the exit of their α-tanycyte descendants from the germinal 3V wall. Our findings suggest that transience of cells through the α-tanycyte domain is a key feature, and Fgf10 is a negative regulator of postnatal hypothalamic neurogenesis.

Item Type:	Article
Uncontrolled Keywords:	cell movement,fibroblast growth factors,hypothalamus,lineage tracing,mouse,postnatal neurogenesis,tanycytes,molecular biology,developmental biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Cells and Tissues
Related URLs:	http://www.scopus.com/inward/record.url?... http://dev.biologists.org/lookup/doi/10....
Depositing User:	LivePure Connector
Date Deposited:	22 Jul 2020 02:46
Last Modified:	23 Apr 2025 00:13
URI:	https://ueaeprints.uea.ac.uk/id/eprint/76216
DOI:	10.1242/dev.180950

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