HT-SPOTi: A rapid drug susceptibility test (DST) to evaluate antibiotic resistance profiles and novel chemicals for anti-infective drug discovery

Danquah, Cynthia A., Maitra, Arundhati, Gibbons, Simon, Faull, Jane and Bhakta, Sanjib (2016) HT-SPOTi: A rapid drug susceptibility test (DST) to evaluate antibiotic resistance profiles and novel chemicals for anti-infective drug discovery. Current Protocols in Microbiology, 2016. 17.8.1-17.8.12. ISSN 1934-8525

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Abstract

Antibiotic resistance is one of the major threats to global health and wellbeing. The past decade has seen an alarming rise in the evolution and spread of drug-resistant strains of pathogenic microbes. The emergence of extensively drug resistant (XDR) strains of Mycobacterium tuberculosis and antimicrobial resistance among the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, andEnterobacter species) as well as fungal pathogens (such as certain species of Candida, Aspergillus, Cryptococcus, and Trichophyton) poses a significant 21st century scientific challenge. With an extremely limited arsenal of efficacious antibiotics, techniques that can (a) identify novel antimicrobials and (b) detect antimicrobial resistance are becoming increasingly important. In this article, we illustrate the HT-SPOTi, an assay that is principally based on the growth of an organism on agar medium containing a range of different concentrations of drugs or inhibitors. The simple methodology makes this assay ideal for evaluating novel antimicrobial compounds as well as profiling an organism’s antibiotic resistance profile.

Item Type: Article
Uncontrolled Keywords: antimicrobial resistance,diagnosis,drug discovery,drug susceptibility testing (dst),ht-spoti,infectious diseases,whole-cell phenotypic evaluation,parasitology,microbiology,virology ,/dk/atira/pure/subjectarea/asjc/2400/2405
Faculty \ School: Faculty of Science > School of Pharmacy
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 14 Jul 2020 23:51
Last Modified: 14 Jul 2020 23:51
URI: https://ueaeprints.uea.ac.uk/id/eprint/76050
DOI: 10.1002/9780471729259.mc1708s40

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