Flavonoids from Artemisia rupestris and their synergistic antibacterial effects on drug-resistant Staphylococcus aureus

Lan, Jiang-Er, Li, Xiao-Jin, Zhu, Xiao-Feng, Sun, Zhong-Lin, He, Jian-Ming, Zloh, Mire, Gibbons, Simon and Mu, Qing (2021) Flavonoids from Artemisia rupestris and their synergistic antibacterial effects on drug-resistant Staphylococcus aureus. Natural Product Research, 35 (11). pp. 1881-1886. ISSN 1478-6419

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Abstract

This study seeks to discover flavonoids from a traditional Chinese herb, Artemisia rupestris L., with synergistic antibacterial effects against multidrug-resistant Staphylococcus aureus. Five flavonoids, artemetin (1), chrysosplenetin (2), pachypodol (3), penduletin (4) and chrysoeriol (5) were obtained by various column chromatographic methods. Their chemical structures were determined on the basis of comprehensive spectroscopic analysis and comparison with literature data. Three of the compounds (2, 4 and 5) exhibited synergistic activity when combined with norfloxacin against SA1199B, an effluxing fluoroquinolone-resistant strain. The fractional inhibitory concentration indices (FICIs) of 2, 4 and 5 in combination with norfloxacin were 0.375, 0.079 and 0.266 respectively, suggesting synergy. Compound 5 also showed synergistic effects against EMRSA-15 and EMRSA-16 when combined with ciprofloxacin and oxacillin exhibiting FICIs of 0.024 and 0.375 respectively. Real time ethidium bromide (EtBr) efflux assay, qRT-PCR and molecular docking were employed to explore the mechanisms of the synergistic effects.

Item Type: Article
Uncontrolled Keywords: artemisia rupestrisl,efflux inhibitor,flavonoids,molecular docking,multidrug-resistant staphylococcus aureus,synergism,analytical chemistry,biochemistry,plant science,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602
Faculty \ School: Faculty of Science > School of Pharmacy
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Depositing User: LivePure Connector
Date Deposited: 19 Jun 2020 00:05
Last Modified: 30 Sep 2021 15:05
URI: https://ueaeprints.uea.ac.uk/id/eprint/75666
DOI: 10.1080/14786419.2019.1639182

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