The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study

Skalicka-Woźniak, Krystyna, Walasek, Magdalena, Aljarba, Tariq M., Stapleton, Paul, Gibbons, Simon, Xiao, Jianbo and Łuszczki, Jarogniew J. (2018) The anticonvulsant and anti-plasmid conjugation potential of Thymus vulgaris chemistry: An in vivo murine and in vitro study. Food and Chemical Toxicology, 120. pp. 472-478. ISSN 0278-6915

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Abstract

The high-performance counter-current chromatography was used for the efficient purification of single constituents from Thymus vulgaris essential oil. Mixtures of n-heptane, ethyl acetate, methanol, and water (5:2:5:2 and 4:1:4:1 v/v), allowed purification of eugenol, 1-octen-3- ol, borneol, thymol, terpinen-4-ol, and camphor, while n-hexane, acetonitrile, and tert-butyl methyl ether (1:1:0.1 v/v) yielded carvacrol, borneol, linalyl acetate, caryophyllene oxide, p-cymene, and eucalyptol. The anticonvulsant activities were evaluated in the maximal electroshock-induced seizure test in mice model (systemic i. p. administration). The oil exerted protection against MES-induced seizures when administered 15 and 30 min before the tests (50 and 62.5%, respectively). Among the isolates, borneol, thymol, and eugenol exerted the strongest protection against seizures. Moreover, linalool had the ability to reduce the transfer of the pKM101 plasmid by 84%, what has the potential to reduce virulence and resistance spread in E. coli. No acute toxic effects towards the CNS were noticed either for the essential oil or for single compounds, in the chimney and grip-strength tests. The preclinical screening of Thymus vulgaris EO, as well as isolated terpenoids, provides evidence that the EO has partial protective activity against seizures and HPCCC technique is suitable for its large scale isolation.

Item Type: Article
Uncontrolled Keywords: anti-conjugation,epilepsy,escherichia coli,isolation,natural products,purification,seizure,terpenes,food science,toxicology ,/dk/atira/pure/subjectarea/asjc/1100/1106
Faculty \ School: Faculty of Science > School of Pharmacy
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 18 Jun 2020 00:02
Last Modified: 18 Jun 2020 00:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/75644
DOI: 10.1016/j.fct.2018.07.045

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