Fluorescent amino acids as versatile building blocks for chemical biology

Cheng, Zhiming, Kuru, Erkin, Sachdeva, Amit and Vendrell, Marc (2020) Fluorescent amino acids as versatile building blocks for chemical biology. Nature Reviews Chemistry, 4. pp. 275-290. ISSN 2397-3358

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Fluorophores have transformed the way we study biological systems, enabling non-invasive studies in cells and intact organisms, which increase our understanding of complex processes at the molecular level. Fluorescent amino acids have become an essential chemical tool because they can be used to construct fluorescent macromolecules, such as peptides and proteins, without disrupting their native biomolecular properties. Fluorescent and fluorogenic amino acids with unique photophysical properties have been designed for tracking protein–protein interactions in situ or imaging nanoscopic events in real time with high spatial resolution. In this Review, we discuss advances in the design and synthesis of fluorescent amino acids and how they have contributed to the field of chemical biology in the past 10 years. Important areas of research that we review include novel methodologies to synthesize building blocks with tunable spectral properties, their integration into peptide and protein scaffolds using site-specific genetic encoding and bioorthogonal approaches, and their application to design novel artificial proteins, as well as to investigate biological processes in cells by means of optical imaging. [Figure not available: see fulltext.].

Item Type: Article
Uncontrolled Keywords: chemistry(all),chemical engineering(all) ,/dk/atira/pure/subjectarea/asjc/1600
Faculty \ School: Faculty of Science > School of Chemistry
UEA Research Groups: Faculty of Science > Research Groups > Chemistry of Life Processes
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Depositing User: LivePure Connector
Date Deposited: 18 Jun 2020 00:01
Last Modified: 22 Oct 2022 06:18
URI: https://ueaeprints.uea.ac.uk/id/eprint/75634
DOI: 10.1038/s41570-020-0186-z


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