A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene

Gardiner, Laura Jayne, Bansept-Basler, Pauline, El-Soda, Mohamed, Hall, Anthony and O'Sullivan, Donal M. (2020) A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene. PLoS One, 15 (4). ISSN 1932-6203

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Abstract

We used three approaches to map the yellow rust resistance gene Yr7 and identify associated SNPs in wheat. First, we used a traditional QTL mapping approach using a double haploid (DH) population and mapped Yr7 to a low-recombination region of chromosome 2B. To fine map the QTL, we then used an association mapping panel. Both populations were SNP array genotyped allowing alignment of QTL and genome-wide association scans based on common segregating SNPs. Analysis of the association panel spanning the QTL interval, narrowed the interval down to a single haplotype block. Finally, we used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. We highlight the power of combining mapping-by-sequencing, delivering a complete list of gene-based segregating polymorphisms in the interval with the high recombination, low LD precision of the association mapping panel. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.

Item Type: Article
Uncontrolled Keywords: biochemistry, genetics and molecular biology(all),agricultural and biological sciences(all),general ,/dk/atira/pure/subjectarea/asjc/1300
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 25 Apr 2020 00:03
Last Modified: 17 Jul 2020 23:44
URI: https://ueaeprints.uea.ac.uk/id/eprint/74834
DOI: 10.1371/journal.pone.0231157

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