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Gardiner, Laura Jayne, Bansept-Basler, Pauline, El-Soda, Mohamed, Hall, Anthony 
ORCID: https://orcid.org/0000-0002-1806-020X and O'Sullivan, Donal M.
(2020)
A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene.
PLoS One, 15 (4).
ISSN 1932-6203
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Abstract
We used three approaches to map the yellow rust resistance gene Yr7 and identify associated SNPs in wheat. First, we used a traditional QTL mapping approach using a double haploid (DH) population and mapped Yr7 to a low-recombination region of chromosome 2B. To fine map the QTL, we then used an association mapping panel. Both populations were SNP array genotyped allowing alignment of QTL and genome-wide association scans based on common segregating SNPs. Analysis of the association panel spanning the QTL interval, narrowed the interval down to a single haplotype block. Finally, we used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. We highlight the power of combining mapping-by-sequencing, delivering a complete list of gene-based segregating polymorphisms in the interval with the high recombination, low LD precision of the association mapping panel. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | biochemistry, genetics and molecular biology(all),agricultural and biological sciences(all),general ,/dk/atira/pure/subjectarea/asjc/1300 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 25 Apr 2020 00:03 |
| Last Modified: | 22 Oct 2022 06:03 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/74834 |
| DOI: | 10.1371/journal.pone.0231157 |
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