The crystal structure of a biological insulated transmembrane molecular wire

Edwards, Marcus, White, Gaye, Butt, Julea, Richardson, David and Clarke, Tom (2020) The crystal structure of a biological insulated transmembrane molecular wire. Cell, 181 (3). 665-673.e10. ISSN 0092-8674

Preview	PDF (CELL-D-19-02331_R3_removed) - Accepted Version Available under License Creative Commons Attribution. Download (2MB) | Preview
Preview	PDF (Published_Version) Available under License Creative Commons Attribution. Download (5MB) | Preview

Abstract

A growing number of bacteria are recognized to conduct electrons across their cell envelope, and yet molecular details of the mechanisms supporting this process remain unknown. Here, we report the atomic structure of an outer membrane spanning protein complex, MtrAB, that is representative of a protein family known to transport electrons between the interior and exterior environments of phylogenetically and metabolically diverse microorganisms. The structure is revealed as a naturally insulated biomolecular wire possessing a 10-heme cytochrome, MtrA, insulated from the membrane lipidic environment by embedding within a 26 strand β-barrel formed by MtrB. MtrAB forms an intimate connection with an extracellular 10-heme cytochrome, MtrC, which presents its hemes across a large surface area for electrical contact with extracellular redox partners, including transition metals and electrodes.

Item Type:	Article
Uncontrolled Keywords:	decaheme,electrogenic bacteria,extracellular electron transfer,geobacter,iron oxidation,metal reduction,outer membrane protein,porin-cytochrome complex,respiration,shewanella,biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/1300
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science > School of Natural Sciences (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Science > Research Groups > Wolfson Centre for Advanced Environmental Microbiology
Related URLs:	https://www.rcsb.org/structure/6R2Q https://www.rcsb.org/structure/6QYC
Depositing User:	LivePure Connector
Date Deposited:	25 Mar 2020 01:33
Last Modified:	28 Jun 2025 00:41
URI:	https://ueaeprints.uea.ac.uk/id/eprint/74613
DOI:	10.1016/j.cell.2020.03.032

Downloads

Actions (login required)

View Item