1,2-diacylglycerol kinase of human erythrocyte membranes. Assay with endogenously generated substrate

ALLAN, D, THOMAS, P and GATT, S (1980) 1,2-diacylglycerol kinase of human erythrocyte membranes. Assay with endogenously generated substrate. Biochemical Journal, 191 (2). 669–672. ISSN 0264-6021

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Abstract

1,2-Diacylglycerol kinase activity was measured in human erythrocyte membranes using an assay procedure in which the substrate was generated endogenously, either by treatment with a bacterial phospholipase C or by incubation with Ca24, which activates a membrane-bound polyphosphoinositide phosphodiesterase. The properties of 1,2-diacylglycerol kinase were broadly similar to those described previously, except that in the present work maximum activities were higher and there was evidence for a double pH optimum.

Item Type: Article
Faculty \ School: Faculty of Science
Depositing User: LivePure Connector
Date Deposited: 03 Mar 2020 09:10
Last Modified: 22 Apr 2020 08:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/74392
DOI: 10.1042/bj1910669

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