FilTar: Using RNA-Seq data to improve microRNA target prediction accuracy in animals

Bradley, Thomas and Moxon, Simon ORCID: (2020) FilTar: Using RNA-Seq data to improve microRNA target prediction accuracy in animals. Bioinformatics, 36 (8). 2410–2416. ISSN 1367-4803

[thumbnail of Published_Version]
PDF (Published_Version) - Published Version
Available under License Creative Commons Attribution.

Download (707kB) | Preview


MOTIVATION: MicroRNA (miRNA) target prediction algorithms do not generally consider biological context and therefore generic target prediction based on seed binding can lead to a high level of false-positive predictions. Here, we present FilTar, a method that incorporates RNA-Seq data to make miRNA target prediction specific to a given cell type or tissue of interest. RESULTS: We demonstrate that FilTar can be used to: (i) provide sample specific 3'-UTR reannotation; extending or truncating default annotations based on RNA-Seq read evidence and (ii) filter putative miRNA target predictions by transcript expression level, thus removing putative interactions where the target transcript is not expressed in the tissue or cell line of interest. We test the method on a variety of miRNA transfection datasets and demonstrate increased accuracy versus generic miRNA target prediction methods. AVAILABILITY AND IMPLEMENTATION: FilTar is freely available and can be downloaded from The tool is implemented using the Python and R programming languages, and is supported on GNU/Linux operating systems. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 03 Jan 2020 04:00
Last Modified: 22 Oct 2022 05:38
DOI: 10.1093/bioinformatics/btaa007


Downloads per month over past year

Actions (login required)

View Item View Item