Quercetin exposure suppresses the inflammatory pathway in intestinal organoids from winnie mice

Dicarlo, Manuela, Teti, Gabriella, Verna, Giulio, Liso, Marina, Cavalcanti, Elisabetta, Sila, Annamaria, Raveenthiraraj, Sathuwarman, Mastronardi, Mauro, Santino, Angelo, Serino, Grazia, Lippolis, Antonio, Sobolewski, Anastasia, Falconi, Mirella and Chieppa, Marcello (2019) Quercetin exposure suppresses the inflammatory pathway in intestinal organoids from winnie mice. International Journal of Molecular Sciences, 20 (22). ISSN 1661-6596

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Abstract

Inflammatory bowel diseases (IBDs) are chronic and relapsing immune disorders that result, or possibly originate, from epithelial barrier defects. Intestinal organoids are a new reliable tool to investigate epithelial response in models of chronic inflammation. We produced organoids from the ulcerative colitis murine model Winnie to explore if the chronic inflammatory features observed in the parental intestine were preserved by the organoids. Furthermore, we investigated if quercetin administration to in vitro cultured organoids could suppress LPS-induced inflammation in wild-type organoids (WT-organoids) and spontaneous inflammation in ulcerative colitis organoids (UC-organoids). Our data demonstrate that small intestinal organoids obtained from Winnie mice retain the chronic intestinal inflammatory features characteristic of the parental tissue. Quercetin administration was able to suppress inflammation both in UC-organoids and in LPS-treated WT-organoids. Altogether, our data demonstrate that UC-organoids are a reliable experimental system for investigating chronic intestinal inflammation and pharmacological responses.

Item Type: Article
Uncontrolled Keywords: inflammatory bowel diseases,quercetin,small intestinal organoids,catalysis,molecular biology,spectroscopy,computer science applications,physical and theoretical chemistry,organic chemistry,inorganic chemistry ,/dk/atira/pure/subjectarea/asjc/1500/1503
Faculty \ School: Faculty of Science > School of Pharmacy
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Depositing User: LivePure Connector
Date Deposited: 30 Nov 2019 02:05
Last Modified: 17 Oct 2020 23:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/73221
DOI: 10.3390/ijms20225771

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