Aptamer-modified gold nanoparticles for rapid aggregation-based detection of inflammation: an optical assay for interleukin-6

Giorgi-Call, Susan, Marin, Maria J., Sule, Olajumoke, Hutchinson, Peter J. and Carpenter, Keri L. H. (2019) Aptamer-modified gold nanoparticles for rapid aggregation-based detection of inflammation: an optical assay for interleukin-6. Microchimica Acta. ISSN 0026-3672 (In Press)

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Abstract

A proof-of-concept aptamer-based optical assay is described for the determination of the immune signalling molecule interleukin-6 (IL-6), a key marker of acute inflammation. The optical assay is based on the aggregation of gold nanoparticles (AuNP) coated in two complimentary “sandwich-style” aptamers, each with different IL-6 target moieties. IL-6 will recognise the complimentary aptamer pair and bind to it causing the aggregation of the corresponding functionalised nanoparticles. The aggregation of the AuNPs after exposure to IL-6 induces a visible colour change from red to pink, with a corresponding change in the extinction maximum from 520 to 540 nm. The change in the extinction maximum can be monitored visually, or using a spectrophotometer or a plate reader. The optimal size and functionalisation method of aptamer-coated AuNP, and potential assay format were investigated using UV-Vis spectrophotometry, transmission electron microscopy, and dynamic light scattering. The optical assay was applied for detecting mouse IL-6 in a mixed protein solution as a representative biological sample. The assay works in the 3.3 to 125 µg·mL-1 IL6 concentration range, and the detection limit (at S/N = 3) is 1.95 µg·mL-1. This study was performed as a proof-of-concept demonstration of this versatile assay design, with a view to developing a similar assay for use in clinical samples in future.

Item Type: Article
Uncontrolled Keywords: cytokines,colorimetric,metal nanoparticles,sepsis,diagnostics,point-of-care assay
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science
Depositing User: LivePure Connector
Date Deposited: 31 Oct 2019 15:42
Last Modified: 31 Oct 2019 15:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/72824
DOI:

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