A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana

Van de Weyer, Anna-Lena, Monteiro, Freddy, Furzer, Oliver J, Nishimura, Marc T, Cevik, Volkan, Witek, Kamil, Jones, Jonathan D G, Dangl, Jeffery L, Weigel, Detlef and Bemm, Felix (2019) A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana. Cell, 178 (5). 1260-1272.e14. ISSN 0092-8674

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Abstract

Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consistent with extensive balancing and positive selection, NLRs are encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define a nearly complete species-wide pan-NLRome in Arabidopsis thaliana based on sequence enrichment and long-read sequencing. The pan-NLRome largely saturates with approximately 40 well-chosen wild strains, with half of the pan-NLRome being present in most accessions. We chart NLR architectural diversity, identify new architectures, and quantify selective forces that act on specific NLRs and NLR domains. Our study provides a blueprint for defining pan-NLRomes.

Item Type: Article
Additional Information: Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
Faculty \ School:
Faculty of Science > School of Biological Sciences
Depositing User: LivePure Connector
Date Deposited: 15 Oct 2019 12:30
Last Modified: 20 Aug 2020 00:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/72600
DOI: 10.1016/j.cell.2019.07.038

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