A biotechnological approach to understanding the interactions between endothelial cell integrins and neuropilin-2 in Angiogenesis

Alghamdi, Abdullah (2019) A biotechnological approach to understanding the interactions between endothelial cell integrins and neuropilin-2 in Angiogenesis. Doctoral thesis, University of East Anglia.

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Abstract

Although a number of studies have focused on the role of neuropilin-2 (NRP2) in early embryological development and in various cancer cells, very little is known about the biological roles of NRP2 in microvascular endothelial cells (ECs). Here, we show that siRNA depletion of NRP2 decreases fibronectin (FN)-dependent migration, adhesion and focal adhesion assembly and disassembly in murine lung microvascular endothelial cells (ECs). Compared to control cells, NRP2-depleted cells exhibit elevated levels of total cellular integrin-α5 (ITGA5 - the main FN receptor in ECs), but this increase does not translate to the cell surface. MS analysis identified the major FN receptors α5β1 as NRP2 binding partners. However, ITGA5 internalization and recycling assays revealed that NRP2 knockdown suppress ITGA5 recycling to the cell surface and a direct interaction between NRP2 and ITGA5 is observed by co-immunoprecipitation. This suggests that NRP2 controls microvascular EC behaviour on FN by regulating ITGA5 function.

Item Type: Thesis (Doctoral)
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Jackie Webb
Date Deposited: 14 Jun 2019 13:26
Last Modified: 14 Jun 2019 13:26
URI: https://ueaeprints.uea.ac.uk/id/eprint/71398
DOI:

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