Genomic epidemiology of NDM-1-encoding plasmids in latin American clinical isolates reveals insights into the evolution of multidrug resistance

Marquez-Ortiz, Ricaurte Alejandro, Haggerty, Leanne, Olarte, Narda, Duarte, Carolina, Garza-Ramos, Ulises, Silva-Sanchez, Jesus, Castro, Betsy E., Sim, Eby M., Beltran, Mauricio, Moncada, María V., Valderrama, Alberto, Castellanos, Jaime E., Charles, Ian G., Vanegas, Natasha, Escobar-Perez, Javier and Petty, Nicola K. (2017) Genomic epidemiology of NDM-1-encoding plasmids in latin American clinical isolates reveals insights into the evolution of multidrug resistance. Genome Biology and Evolution, 9 (6). pp. 1725-1741. ISSN 1759-6653

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Abstract

Bacteria that produce the broad-spectrum Carbapenem antibiotic NewDelhi Metallo-b-lactamase (NDM) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistancemechanism.Although it is believed that theassociated resistancegenebla NDM-1 originated inAcinetobacter spp., the role of Enterobacteriaceae in its dissemination remains unclear. In this study, we usedwhole genome sequencing to investigate the dissemination dynamics of blaNDM-1-positive plasmids in a set of 21 clinical NDM-1-positive isolates from Colombia and Mexico (Providencia rettgeri, Klebsiella pneumoniae, and Acinetobacter baumannii) aswell as six representative NDM-1-positive Escherichia coli transconjugants. Additionally, the plasmids from three representative P. rettgeri isolates were sequenced by PacBio sequencing and finished. Our results demonstrate the presence of previously reported plasmids from K. pneumoniae and A. baumannii in different genetic backgrounds and geographically distant locations in Colombia. Three new previously unclassified plasmids were also identified in P. rettgeri from Colombia and Mexico, plus an interesting genetic link between NDM-1-positive P. rettgeri from distant geographic locations (Canada, Mexico, Colombia, and Israel) without any reported epidemiological links was discovered. Finally, we detected a relationship between plasmids present in P. rettgeri and plasmids from A. baumannii and K. pneumoniae. Overall, our findings suggest a Russian dollmodel for the dissemination of blaNDM-1 in LatinAmerica,with P. rettgeri playing a central role in this process, andrevealnewinsights into the evolution and disseminationof plasmids carrying such antibiotic resistance genes.

Item Type: Article
Uncontrolled Keywords: antibiotic resistance,bacterial evolution,genomics,metallo-beta-lactamase,mobile genetic elements,providencia rettgeri,ecology, evolution, behavior and systematics,genetics ,/dk/atira/pure/subjectarea/asjc/1100/1105
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Depositing User: LivePure Connector
Date Deposited: 11 Jun 2019 15:30
Last Modified: 11 Jun 2020 01:09
URI: https://ueaeprints.uea.ac.uk/id/eprint/71316
DOI: 10.1093/gbe/evx115

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