Multiple transmissible genes encoding fluoroquinolone and third-generation cephalosporin resistance co-located in non-typhoidal Salmonella isolated from food-producing animals in China

Jiang, Hong Xia, Song, Li, Liu, Ji, Zhang, Xiao Hua, Ren, Yan Na, Zhang, Wen Hui, Zhang, Jing Yuan, Liu, Ya Hong, Webber, Mark A., Ogbolu, David O., Zeng, Zhen Ling and Piddock, Laura J.V. (2014) Multiple transmissible genes encoding fluoroquinolone and third-generation cephalosporin resistance co-located in non-typhoidal Salmonella isolated from food-producing animals in China. International Journal of Antimicrobial Agents, 43 (3). pp. 242-247. ISSN 0924-8579

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Abstract

The aim of this study was to identify genes conferring resistance to fluoroquinolones and extended-spectrum β-lactams in non-typhoidal Salmonella (NTS) from food-producing animals in China. In total, 31 non-duplicate NTS were obtained from food-producing animals that were sick. Isolates were identified and serotyped and the genetic relatedness of the isolates was determined by pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA. Antimicrobial susceptibility was determined using Clinical and Laboratory Standards Institute methodology. The presence of extended-spectrum β-lactamase (ESBL) and fluoroquinolone resistance genes was established by PCR and sequencing. Genes encoded on transmissible elements were identified by conjugation and transformation. Plasmids were typed by PCR-based replicon typing. The occurrence and diversity of numerous different transmissible genes conferring fluoroquinolone resistance [qnrA, qnrD, oqxA and aac(6′)-Ib-cr] and ESBLs (CTX-M-27 and CTX-M-14), and which co-resided in different isolates and serovars of Salmonella, were much higher than in European countries. Furthermore, different plasmids encoded fluoroquinolone resistance (ca. 6 kb) and β-lactam resistance (ca. 63 kb) and these co-resided in isolates with mutations in topoisomerase genes (gyrA and parC) giving very resistant Salmonella. The presence of multidrug-resistant bacteria in food-producing animals in countries that export foodstuffs suggests that global transfer of antibiotic resistances from country to country on food is possible.

Item Type: Article
Uncontrolled Keywords: esbl,fluoroquinolone,plasmid,salmonella,microbiology (medical),infectious diseases,pharmacology (medical) ,/dk/atira/pure/subjectarea/asjc/2700/2726
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Depositing User: LivePure Connector
Date Deposited: 22 Mar 2019 09:30
Last Modified: 16 Sep 2019 08:38
URI: https://ueaeprints.uea.ac.uk/id/eprint/70301
DOI: 10.1016/j.ijantimicag.2013.12.005

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