Antimicrobial garlic-derived diallyl polysulfanes:Interactions with biological thiols in Bacillus subtilis

Arbach, Miriam, Santana, Taris M., Moxham, Hazel, Tinson, Ryan, Anwar, Awais, Groom, Murree and Hamilton, Christopher (2019) Antimicrobial garlic-derived diallyl polysulfanes:Interactions with biological thiols in Bacillus subtilis. Biochimica et Biophysica Acta - General Subjects, 1863 (6). pp. 1050-1058. ISSN 0304-4165

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Abstract

Background Diallylpolysulfanes are the key constituents of garlic oils, known to exhibit broad spectrum anticancer and antimicrobial activity. Studies in vitro, and in mammalian cells, have shown they react, via thiol-polysulfane exchange, with their major low molecular weight thiol, glutathione. However, there are no detailed reports of diallylpolysulfane effects on other common thiol metabolites (cysteine and coenzyme A) or major thiol cofactors (e.g. bacillithiol) that many Gram positive bacteria produce instead of glutathione. Methods Diallylpolysulfanes were individually purified then screened for antimicrobial activity against Bacillus subtilis. Their impact on thiol metabolites (bacillithiol, cysteine, coenzyme A, protein thiols allyl thiols//persulfides) in B. subtilis cultures were analysed, by HPLC. Results Diallylpolysulfane bioactivity increased with increasing chain length up to diallyltetrasulfane, but then plateaued. Within two minutes of treating B. subtilis with diallyltrisulfane or diallyltetrasulfane intracellular bacillithiol levels decreased by ~90%. Cysteine and CoA were also affected but to a lesser degree. This was accompanied by the accumulation of allyl thiol and allyl persulfide. A significant level of protein-S-allylation was also detected. Conclusions In addition to the major low molecular weight thiol, diallylpolysulfanes can also have an impact on other thiol metabolites and protein thiols. General significance This study shows the rapid parallel impact of polysulfanes on different biological thiols inside Bacillus subtilis alongside the concomitant generation of allyl thiols and persulfides.

Item Type: Article
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Depositing User: LivePure Connector
Date Deposited: 21 Mar 2019 13:26
Last Modified: 16 Sep 2020 23:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/70293
DOI: 10.1016/j.bbagen.2019.03.012

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