Osteotropic agents regulate the expression of osteoclast differentiation factor and osteoprotegerin in osteoblastic stromal cells

Horwood, N J, Elliott, Jan, Martin, T J and Gillespie, M T (1998) Osteotropic agents regulate the expression of osteoclast differentiation factor and osteoprotegerin in osteoblastic stromal cells. Endocrinology, 139 (11). pp. 4743-4746. ISSN 0013-7227

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Abstract

The osteoclastogenic factor of osteoblastic origin has recently been elucidated as a novel Tumor Necrosis Factor (TNF)-ligand family member, termed osteoclast differentiation factor (ODF). Using a semiquantitative RT-PCR approach, we sought to determine the mRNA expression of ODF and its decoy receptor, osteoprotegerin (OPG), in a selection of osteoblastic cell lines and in response to three factors representative of different signal transduction pathways, vitamin D receptor, protein kinase A or gp130. Each osteotropic agent, either 1,25-(OH)2D3, PTH or IL-11, promoted an increase in the ratio of ODF:OPG, with maximal stimulation occurring at 24 h, 4 h, and 8 h, respectively, and furthermore each was shown to act in a dose-dependent manner. This report establishes that osteoblastic cell lines incapable of supporting osteoclast formation have markedly reduced ODF expression and also illustrates the importance of the relative abundance of ODF compared with the levels of OPG for the induction of osteoclastogenesis.

Item Type: Article
Uncontrolled Keywords: animals,animals, newborn,pharmacology,carrier proteins,cells, cultured,physiology,biosynthesis,pharmacology,pharmacology,biosynthesis,mice,mice, inbred c57bl,drug effects,drug effects,osteoprotegerin,pharmacology,rank ligand,receptor activator of nuclear factor-kappa b,physiology,receptors, cytoplasmic and nuclear,receptors, tumor necrosis factor,reverse transcriptase polymerase chain reaction,drug effects,drug effects
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 06 Mar 2019 09:30
Last Modified: 22 Apr 2020 07:34
URI: https://ueaeprints.uea.ac.uk/id/eprint/70115
DOI: 10.1210/endo.139.11.6433

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