The glycogen synthase kinase MoGsk1, regulated by Mps1 MAP kinase, is required for fungal development and pathogenicity in Magnaporthe oryzae

Zhou, Tengsheng, Dagdas, Yasin F., Zhu, Xiaohan, Zheng, Shiqin, Chen, Liqiong, Cartwright, Zachary, Talbot, Nicholas J. ORCID: https://orcid.org/0000-0001-6434-7757 and Wang, Zonghua (2017) The glycogen synthase kinase MoGsk1, regulated by Mps1 MAP kinase, is required for fungal development and pathogenicity in Magnaporthe oryzae. Scientific Reports, 7. ISSN 2045-2322

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Abstract

Magnaporthe oryzae, the causal agent of blast disease, is one of the most destructive plant pathogens, causing significant yield losses on staple crops such as rice and wheat. The fungus infects plants with a specialized cell called an appressorium, whose development is tightly regulated by MAPK signaling pathways following the activation of upstream sensors in response to environmental stimuli. Here, we show the expression of the Glycogen synthase kinase 3 (GSK3) MoGSK1 in M. oryzae is regulated by Mps1 MAP kinase, particularly under the stressed conditions. Thus, MoGSK1 is functionally characterized in this study. MoGsk1 is functionally homologues to the Saccharomyces cerevisiae GSK3 homolog MCK1. Gene replacement of MoGSK1 caused significant delay in mycelial growth, complete loss of conidiation and inability to penetrate the host surface by mycelia-formed appressorium-like structures, consequently resulting in loss of pathogenicity. However, the developmental and pathogenic defects of Delta mogsk1 are recovered via the heterologous expression of Fusarium graminearum GSK3 homolog gene FGK3, whose coding products also shows the similar cytoplasmic localization as MoGsk1 does in M. oryzae. By contrast, overexpression of MoGSK1 produced deformed appressoria in M. oryzae. In summary, our results suggest that MoGsk1, as a highly conservative signal modulator, dictates growth, conidiation and pathogenicity of M. oryzae.

Item Type:	Article
Uncontrolled Keywords:	rice blast fungus,protein-kinase,saccharomyces-cerevisiae,transcription factor,plant infection,grisea,yeast,gene,mpg1,encodes
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
Depositing User:	LivePure Connector
Date Deposited:	15 Feb 2019 11:30
Last Modified:	31 Jan 2024 02:18
URI:	https://ueaeprints.uea.ac.uk/id/eprint/69944
DOI:	10.1038/s41598-017-01006-w

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