Free energy profile of domain movement in ligand-free citrate synthase

Roccatano, Danilo and Hayward, Steven ORCID: (2019) Free energy profile of domain movement in ligand-free citrate synthase. Journal of Physical Chemistry B, 123 (9). 1998–2004. ISSN 1520-6106

[thumbnail of Accepted_Manuscript]
PDF (Accepted_Manuscript) - Accepted Version
Download (970kB) | Preview


Citrate synthase plays a fundamental role in the metabolic cycle of the cell. Its catalytic mechanism is complex involving the binding of two substrates that cause a domain movement. In this paper, we used classical molecular dynamics simulations and umbrella sampling simulations to determine the potential of mean force along a reaction coordinate for the domain movement in ligand-free citrate synthase from pig (Sus Scrofa). The results show that at 293 K, the closed-domain conformation has a ~4 kbT higher energy than the open-domain conformation. In a simple two-state model, this difference means that the enzyme spends 98% of the time in the open-domain conformation ready to receive the substrate, oxaloacetate, rather than the closed-domain conformation where the binding site would be inaccessible to the substrate. Given that experimental evidence indicates that the binding of oxaloacetate induces at least partial closure, this would imply an induced-fit mechanism which we argue is applicable to all enzymes with a functional domain movement for reasons of catalytic efficiency. A barrier of 4 kbT gives an estimation of the mean first passage time in the range 1-10 μs.

Item Type: Article
Faculty \ School: Faculty of Science > School of Computing Sciences
UEA Research Groups: Faculty of Science > Research Groups > Computational Biology
Depositing User: LivePure Connector
Date Deposited: 15 Feb 2019 11:30
Last Modified: 20 Apr 2023 23:58
DOI: 10.1021/acs.jpcb.8b12001


Downloads per month over past year

Actions (login required)

View Item View Item