Two independent S-phase checkpoints regulate appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae

Oses-Ruiz, Mariam, Sakulkoo, Wasin, Littlejohn, George R., Martin-Urdiroz, Magdalena and Talbot, Nicholas J. (2017) Two independent S-phase checkpoints regulate appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae. Proceedings of the National Academy of Sciences, 114 (2). E237-E244. ISSN 0027-8424

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Abstract

To cause rice blast disease, the fungal pathogen Magnaporthe oryzae develops a specialized infection structure called an appressorium. This dome-shaped, melanin-pigmented cell generates enormous turgor and applies physical force to rupture the rice leaf cuticle using a rigid penetration peg. Appressorium-mediated infection requires septin-dependent reorientation of the F-actin cytoskeleton at the base of the infection cell, which organizes polarity determinants necessary for plant cell invasion. Here, we show that plant infection by M. oryzae requires two independent S-phase cell-cycle checkpoints. Initial formation of appressoria on the rice leaf surface requires an S-phase checkpoint that acts through the DNA damage response (DDR) pathway, involving the Cds1 kinase. By contrast, appressorium repolarization involves a novel, DDR-independent S-phase checkpoint, triggered by appressorium turgor generation and melanization. This second checkpoint specifically regulates septin- dependent, NADPH oxidase-regulated F-actin dynamics to organize the appressorium pore and facilitate entry of the fungus into host tissue.

Item Type: Article
Uncontrolled Keywords: fungi,pathogen,pyricularia,appressorium,cell cycle,aspergillus-nidulans,dna-damage,cell-cycle,grisea,penetration,growth,biology,kinase,yeast,pathogenicity
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Depositing User: LivePure Connector
Date Deposited: 15 Feb 2019 10:30
Last Modified: 06 May 2020 23:58
URI: https://ueaeprints.uea.ac.uk/id/eprint/69940
DOI: 10.1073/pnas.1611307114

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