Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions
Walden, Grace, Liao, Xin, Riley, Graham, Donell, Simon, Raxworthy, Michael J. and Saeed, Aram (2019) Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions. Bioconjugate Chemistry, 30 (3). 531–535. ISSN 1043-1802
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Abstract
The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.
Item Type: | Article |
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Uncontrolled Keywords: | biotechnology,bioengineering,biomedical engineering,pharmacology,pharmaceutical science,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1305 |
Faculty \ School: | Faculty of Science > School of Pharmacy Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 12 Feb 2019 16:30 |
Last Modified: | 02 Mar 2021 00:55 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/69916 |
DOI: | 10.1021/acs.bioconjchem.8b00868 |
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