Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions

Tools

Walden, Grace, Liao, Xin, Riley, Graham, Donell, Simon, Raxworthy, Michael J. and Saeed, Aram (2019) Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions. Bioconjugate Chemistry, 30 (3). 531–535. ISSN 1043-1802

[img]
Preview
 PDF (Bioconjugate Chemistry Communication_Grace Walden_accepted version) - Submitted Version
Download (1MB) | Preview
[img]
Preview
 PDF (Supplementary Information Bioconjugate Chemistry Communication_Accepted version)
Download (875kB) | Preview
[img]
Preview
 PDF (TOC graphic accepted version)
Download (190kB) | Preview

Abstract

The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

Item Type: Article
Uncontrolled Keywords: biotechnology,bioengineering,biomedical engineering,pharmacology,pharmaceutical science,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 12 Feb 2019 16:30
Last Modified: 24 Nov 2020 01:22
URI: https://ueaeprints.uea.ac.uk/id/eprint/69916
DOI: 10.1021/acs.bioconjchem.8b00868

Actions (login required)

View Item View Item