Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions

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Walden, Grace, Liao, Xin, Riley, Graham, Donell, Simon, Raxworthy, Michael J. and Saeed, Aram (2019) Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions. Bioconjugate Chemistry, 30 (3). 531–535. ISSN 1043-1802

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Abstract

The rapid surface immobilization of protein on monodispersed polyester microcarriers is reported. A model protein, functionalized with a dibenzocyclooctyne core, immobilizes on the surface of azide-terminal polycaprolactone microcarriers within 10 min compared to 12 h for other conjugation techniques, and it is conducted in physiological conditions and in the absence of coupling reagents.

Item Type: Article
Uncontrolled Keywords: biotechnology,bioengineering,biomedical engineering,pharmacology,pharmaceutical science,organic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 12 Feb 2019 16:30
Last Modified: 06 May 2022 03:12
URI: https://ueaeprints.uea.ac.uk/id/eprint/69916
DOI: 10.1021/acs.bioconjchem.8b00868

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