Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences

Wan, Wei-Lin, Zhang, Lisha, Pruitt, Rory, Zaidem, Maricris, Brugman, Rik, Ma, Xiyu, Krol, Elzbieta, Perraki, Artemis, Kilian, Joachim, Grossmann, Guido, Stahl, Mark, Shan, Libo, Zipfel, Cyril, van Kan, Jan A.L., Hedrich, Rainer, Weigel, Detlef, Gust, Andrea A. and Nürnberger, Thorsten (2019) Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences. New Phytologist, 221 (4). pp. 2080-2095. ISSN 0028-646X

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Abstract

Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine‐rich repeat receptor kinases (LRR‐RK) FLS2 and EFR, and the LRR receptor protein (LRR‐RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR‐RK and LRR‐RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP‐mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23‐regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2‐signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP‐type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.

Item Type: Article
Uncontrolled Keywords: arabidopsis,immune receptor,immune signaling comparison,plant immunity,receptor kinase,receptor protein,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
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Depositing User: LivePure Connector
Date Deposited: 12 Oct 2018 11:30
Last Modified: 18 Nov 2020 00:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/68502
DOI: 10.1111/nph.15497

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