Oxidative Stress and NF-κB signaling are involved in LPS induced pulmonary dysplasia in chick embryos

Long, Yun, Wang, Guang, Li, Ke, Zhang, Zongyi, Zhang, Ping, Zhang, Jing, Zhang, Xiaotan, Bao, Yongping, Yang, Xuesong and Wang, Peng-cheng (2018) Oxidative Stress and NF-κB signaling are involved in LPS induced pulmonary dysplasia in chick embryos. Cell Cycle, 17 (14). pp. 1757-1771. ISSN 1538-4101

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Abstract

Inflammation or dysbacteriosis-derived lipopolysaccharides (LPS) adversely influence the embryonic development of respiratory system. However, the precise pathological mechanisms still remain to be elucidated. In this study, we demonstrated that LPS exposure caused lung maldevelopment in chick embryos, including higher embryo mortality, increased thickness of alveolar gas exchange zone, and accumulation of PAS+ immature pulmonary cells, accompanied with reduced expression of alveolar epithelial cell markers and lamellar body count. Upon LPS exposure, pulmonary cell proliferation was significantly altered and cell apoptosis was inhibited as well, indicating a delayed progress of pulmonary development. LPS treatment also resulted in reduced CAV-1 expression and up-regulation of Collagen I, suggesting increased lung fibrosis, which was verified by Masson staining. Moreover, LPS induced enhanced Nrf2 expression in E18 lungs, and the increased reactive oxygen species (ROS) production was confirmed in MLE-12 cells in vitro. Antioxidant vitamin C restored the LPS induced down-regulation of ABCA3, SP-C and GATA-6 in MLE-12 cells. Furthermore, LPS induced activation of NF-κB signaling in MLE-12 cells, and the LPS-induced decrease in SP-C expression was partially abrogated by blocking NF-κB signaling with Bay-11-7082. Bay-11-7082 also inhibited LPS-induced increases of ROS and Nrf2 expression. Taken together, we have demonstrated that oxidative stress and NF-κB signaling are involved in LPS induced disruption of pulmonary cell development in chick embryos.

Item Type: Article
Uncontrolled Keywords: lps,embryonic lung development,oxidative stress,nf-κb signaling,gata-6
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 13 Jul 2018 12:30
Last Modified: 11 Jun 2020 00:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/67622
DOI: 10.1080/15384101.2018.1496743

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