Ambient temperature regulates the expression of a small set of sRNAs influencing plant development through NF‐YA2 and YUC2

Gyula, Péter, Baksa, Ivett, Tóth, Tamás, Mohorianu, Irina, Dalmay, Tamás and Szittya, György (2018) Ambient temperature regulates the expression of a small set of sRNAs influencing plant development through NF‐YA2 and YUC2. Plant, Cell and Environment, 41 (10). pp. 2404-2417. ISSN 0140-7791

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Abstract

Plants substantially alter their developmental program upon changes in the ambient temperature. The 21–24 nt small RNAs (sRNAs) are important gene expression regulators, which play a major role in development and adaptation. However, little is known about how the different sRNA classes respond to changes in the ambient temperature. We profiled the sRNA populations in four different tissues of Arabidopsis thaliana plants grown at 15, 21 and 27 °C. We found that only a small fraction (0.6%) of the sRNA loci are ambient temperature‐controlled. We identified thermoresponsive miRNAs and identified their target genes using degradome libraries. We verified that the target of the thermoregulated miR169, NF‐YA2, is also ambient temperature‐regulated. NF‐YA2, as the component of the conserved transcriptional regulator NF‐Y complex, binds the promoter of the flowering time regulator FT and the auxin biosynthesis gene YUC2. Other differentially expressed loci include thermoresponsive phased siRNA loci that target various auxin pathway genes and tRNA fragments. Furthermore, a temperature dependent 24‐nt heterochromatic siRNA locus in the promoter of YUC2 may contribute to the epigenetic regulation of auxin homeostasis. This holistic approach facilitated a better understanding of the role of different sRNA classes in ambient temperature adaptation of plants.

Item Type: Article
Additional Information: Data availability: The raw sequencing data have been deposited in the SRA database under the identifier SRP117737. The NCBI BioProject identifier is PRJNA407271. The scripts used for the analysis are available at https://github.com/gyulap/thermoregulated_sRNAs.
Uncontrolled Keywords: ambient temperature,arabidopsis thaliana,degradome,mirna,phasirna,sirna,trna fragments,nf‐ya2,yuc2
Faculty \ School: Faculty of Science > School of Computing Sciences
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 04 Jul 2018 12:30
Last Modified: 27 Oct 2020 00:54
URI: https://ueaeprints.uea.ac.uk/id/eprint/67526
DOI: 10.1111/pce.13355

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