Determination of free 25(OH)D concentrations and their relationships to total 25(OH)D in multiple clinical populations

Schwartz, Janice B, Gallagher, Christopher, Jorde, Rolf, Berg, Vivian, Walsh, Jennifer, Eastell, Richard, Evans, Amy L, Bowles, Simon, Naylor, K E, Jones, Kerry S, Schoenmakers, Inez, Holick, Michael, Orwoll, Eric, Nielson, Carrie, Kaufman, Martin, Jones, Glenville, Bouillon, Roger, Lai, Jennifer, Verotta, Davide and Bikle, Daniel (2018) Determination of free 25(OH)D concentrations and their relationships to total 25(OH)D in multiple clinical populations. Journal of Clinical Endocrinology & Metabolism, 103 (9). 3278–3288. ISSN 0021-972X

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Abstract

Context: The optimal measure of vitamin D(D) status is unknown. Objective: Directly measure circulating free 25(OH)D concentrations and relationships to total 25(OH)D in a clinically diverse sample of humans. Design: Cross-sectional analysis Setting: Seven academic sites Patients: 1661 adults: (healthy(n=211), pre-diabetic(n=479), outpatients(n=783), cirrhotic(n=90), pregnant(n=20), nursing home(n=79)) Interventions: Merge research data on circulating free 25(OH)D (directly measured immunoassay), total 25(OH)D (LC/MS/MS), D binding protein (DBP by radial (polyclonal) immunodiffusion assay)), albumin, creatinine, iPTH and DBP haplotype Main outcome measures: Distribution of free 25(OH)D (ANOVA with Bonferroni correction for post hoc comparisons) and relationships between free and total 25(OH)D (mixed effects modeling incorporating clinical condition, DBP haplotype with sex, race, eGFR, BMI and other covariates). Results: Free 25(OH)D was 4.7±1.8 pg/mL (mean ±SD) in healthy and 4.3 ±1.9 pg/mL in outpatients with 0.5-8.1 pg/mL and 0.9-8.1 pg/mL encompassing 95% of healthy and outpatients, respectively. Free 25(OH)D was higher in cirrhotics (7.1 ±3.0 pg/mL, p<.0033) and nursing home residents (7.9± 2.1pg/mL, p<.0033) compared to other groups and differed between whites and blacks (p<.0033) and between DBP haplotypes (p<.0001). Mixed effects modeling of relationships between free and total 25(OH)D identified clinical conditions (cirrhotic>nursing home>prediabetic > outpatient > pregnant), and BMI (lesser effect) as covariates affecting relationships but not eGFR, sex, race or DBP haplotype. Conclusions: Total 25(OH)D, health condition, race and DBP haplotype affected free 25(OH)D, but only health conditions and BMI affected relationships between total and free 25(OH) D. Clinical importance of free 25(OH)D needs to be established in studies assessing outcomes.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 02 Jul 2018 09:30
Last Modified: 20 Aug 2020 00:09
URI: https://ueaeprints.uea.ac.uk/id/eprint/67488
DOI: 10.1210/jc.2018-00295

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