Redox-dependent control of i-Motif DNA structure using copper cations

Abdelhamid, Mahmoud A. S., Fábián, László, MacDonald, Colin J., Cheesman, Myles R., Gates, Andrew J. ORCID: https://orcid.org/0000-0002-4594-5038 and Waller, Zoë A. E. (2018) Redox-dependent control of i-Motif DNA structure using copper cations. Nucleic Acids Research, 46 (12). 5886–5893. ISSN 0305-1048

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Abstract

Previous computational studies have shown that Cu+ can act as a substitute for H+ to support formation of cytosine (C) dimers with similar conformation to the hemi-protonated base pair found in i-motif DNA. Through a range of biophysical methods, we provide experimental evidence to support the hypothesis that Cu+ can mediate C–C base pairing in i-motif DNA and preserve i-motif structure. These effects can be reversed using a metal chelator, or exposure to ambient oxygen in the air that drives oxidation of Cu+ to Cu2+, a comparatively weak ligand. Herein, we present a dynamic and redox-sensitive system for conformational control of an i-motif forming DNA sequence in response to copper cations.

Item Type: Article
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science
Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Chemical Biology and Medicinal Chemistry (former - to 2021)
Depositing User: Pure Connector
Date Deposited: 29 May 2018 10:31
Last Modified: 27 Jul 2023 07:42
URI: https://ueaeprints.uea.ac.uk/id/eprint/67211
DOI: 10.1093/nar/gky390

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