Tools
Toolsvan der Lugt, Benthe, Rusli, Fenni, Lute, Carolien, Lamprakis, Andreas, Salazar, Ethel, Boekschoten, Mark V., Hooiveld, Guido J., Müller, Michael, Vervoort, Jacques, Kersten, Sander, Belzer, Clara, Kok, Dieuwertje E. G. and Steegenga, Wilma T. (2018) Integrative analysis of gut microbiota composition, host colonic gene expression and intraluminal metabolites in aging C57BL/6J mice. Aging, 10 (5). pp. 930-950. ISSN 1945-4589
Preview |
PDF (vanderLugt_etal_Aging_2018)
- Published Version
Available under License Creative Commons Attribution. Download (3MB) | Preview |
Abstract
The aging process is associated with diminished colonic health. In this study, we applied an integrative approach to reveal potential interactions between determinants of colonic health in aging C57BL/6J mice. Analysis of gut microbiota composition revealed an enrichment of various potential pathobionts, including Desulfovibrio spp., and a decline of the health-promoting Akkermansia spp. and Lactobacillus spp. during aging. Intraluminal concentrations of various metabolites varied between ages and we found evidence for an increased gut permeability at higher age. Colonic gene expression analysis suggested that during the early phase of aging (between 6 and 12 months), expression of genes involved in epithelial-to-mesenchymal transition and (re)organization of the extracellular matrix were increased. Differential expression of these genes was strongly correlated with Bifidobacterium spp. During the later phase of aging (between 12 and 28 months), gene expression profiles pointed towards a diminished antimicrobial defense and were correlated with an uncultured Gastranaerophilales spp. This study demonstrates that aging is associated with pronounced changes in gut microbiota composition and colonic gene expression. Furthermore, the strong correlations between specific bacterial genera and host gene expression may imply that orchestrated interactions take place in the vicinity of the colonic wall and potentially mediate colonic health during aging.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | aging,gut microbiota,metabolites,colonic gene expression,host-microbe interactions |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Depositing User: | Pure Connector |
| Date Deposited: | 10 May 2018 11:30 |
| Last Modified: | 17 Apr 2025 00:11 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/67017 |
| DOI: | 10.18632/aging.101439 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |