High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes

Ruiz-Tórtola, Ángela, Prats-Quílez, Francisco, González-Lucas, Daniel, Bañuls, María-José, Maquieira, Ángel, Wheeler, Guy, Dalmay, Tamas, Griol, Amadeu, Hurtado, Juan and García-Rupérez, Jaime (2018) High sensitivity and label-free oligonucleotides detection using photonic bandgap sensing structures biofunctionalized with molecular beacon probes. Biomedical Optics Express, 9 (4). pp. 1717-1727. ISSN 2156-7085

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Abstract

A label-free sensor, based on the combination of silicon photonic bandgap (PBG) structures with immobilized molecular beacon (MB) probes, is experimentally developed. Complementary target oligonucleotides are specifically recognized through hybridization with the MB probes on the surface of the sensing structure. This combination of PBG sensing structures and MB probes demonstrates an extremely high sensitivity without the need for complex PCR-based amplification or labelling methods.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 27 Mar 2018 10:38
Last Modified: 22 Jul 2020 02:06
URI: https://ueaeprints.uea.ac.uk/id/eprint/66611
DOI: 10.1364/BOE.9.001717

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