A nanostructural view of the cell wall disassembly process during fruit ripening and postharvest storage by atomic force microscopy

Posé, Sara, Paniagua, Candelas, Matas, Antonio J., Gunning, A. Patrick, Morris, Victor J., Quesada, Miguel A. and Mercado, José A. (2019) A nanostructural view of the cell wall disassembly process during fruit ripening and postharvest storage by atomic force microscopy. Trends in Food Science & Technology, 87. pp. 47-58. ISSN 0924-2244

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Abstract

Background: The mechanical properties of parenchyma cell walls and the strength and extension of adhesion areas between adjacent cells, jointly with cell turgor, are main determinants of firmness of fleshy fruits. These traits are modified during ripening leading to fruit softening. Cell wall modifications involve the depolymerisation of matrix glycans and pectins, the solubilisation of pectins and the loss of neutral sugars from pectin side chains. These changes weaken the cell walls and increase cell separation, which in combination with a reduction in cell turgor, bring about textural changes. Atomic force microscopy (AFM) has been used to characterize the nanostructure of cell wall polysaccharides during the ripening and postharvest storage of several fruits. This technique allows the imaging of individual polymers at high magnification with minimal sample preparation. Scope and approach: This paper reviews the main features of the cell wall disassembly process associated to fruit softening from a nanostructural point of view, as has been provided by AFM studies. Key findings and conclusions: AFM studies show that pectin size, ramification and complexity is reduced during fruit ripening and storage, and in most cases these changes correlate with softening. Postharvest treatments that improve fruit quality have been proven to preserve pectin structure, suggesting a clear link between softening and pectin metabolism. Nanostructural characterization of cellulose and hemicellulose during ripening has been poorly explored by AFM and the scarce results available are not conclusive. Globally, AFM could be a powerful tool to gain insights about the bases of textural fruit quality in fresh and stored fruits.

Item Type: Article
Uncontrolled Keywords: atomic force microscopy,cell wall,hemicellulose,fruit softening,fruit texture,pectins,postharvest
Faculty \ School: Faculty of Science > School of Chemistry
Related URLs:
Depositing User: Pure Connector
Date Deposited: 19 Feb 2018 14:34
Last Modified: 30 Jul 2020 23:47
URI: https://ueaeprints.uea.ac.uk/id/eprint/66309
DOI: 10.1016/j.tifs.2018.02.011

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