Auler, Markus, Pitzler, Lena, Pöschl, Ernst, Zhou, Zhigang and Brachvogel, Bent (2017) Mimicking angiogenesis in vitro: Three-dimensional co-culture of vascular endothelial cells and perivascular cells in collagen type I gels. BIO-Protocol, 7 (8).
Preview |
PDF (Protocol)
- Published Version
Available under License Creative Commons Attribution. Download (6MB) | Preview |
Abstract
Angiogenesis defines the process of formation of new vascular structures form existing blood vessels, involved during development, repair processes like wound healing but also linked to pathological changes. During angiogenic processes, endothelial cells build a vascular network and recruit perivascular cells to form mature, stable vessels. Endothelial cells and perivascular cells secrete and assemble a vascular basement membrane and interact via close cell-cell contacts. To mimic these processes in vitro we have developed a versatile three-dimensional culture system where perivascular cells (PVC) are co-cultured with human umbilical cord vascular endothelial cells (HUVEC) in a collagen type I gel. This co-culture system can be used to determine biochemical and cellular processes during neoangiogenic events with a wide range of analyses options.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | endothelial cells,perivascular cells,pericytes,angiogenesis,collagen gel |
Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
Related URLs: | |
Depositing User: | Pure Connector |
Date Deposited: | 17 Jan 2018 11:30 |
Last Modified: | 04 Mar 2024 17:38 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/65963 |
DOI: | 10.21769/BioProtoc.2247 |
Downloads
Downloads per month over past year
Actions (login required)
![]() |
View Item |