Identification of active denitrifiers by DNA-Stable Isotope Probing and amplicon sequencing reveals Betaproteobacteria as responsible for attenuation of nitrate contamination in a low impacted aquifer

Bellini, M Inés, Kumaresan, Deepak, Tarlera, Silvana, Murrell, J Colin and Scavino, Ana Fernández (2018) Identification of active denitrifiers by DNA-Stable Isotope Probing and amplicon sequencing reveals Betaproteobacteria as responsible for attenuation of nitrate contamination in a low impacted aquifer. FEMS Microbiology Ecology, 94 (2). ISSN 0168-6496

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Abstract

Groundwater reservoirs constitute important freshwater resources. However, these ecosystems are highly vulnerable to contamination and have to rely on the resident microbiota to attenuate the impact of this contamination. Nitrate is one of the main contaminants found in groundwater and denitrification is the main process that removes the compound. In this study, the response to nutrient load on indigenous microbial communities in groundwater from a low impacted aquifer in Uruguay was evaluated. Denitrification rates were measured in groundwater samples from three different sites with nitrate, acetate and pyrite amendments. Results showed that denitrification is feasible under in situ nitrate and electron donor concentrations, although the lack of readily available organic energy source would limit the attenuation of higher nitrate concentrations. DNA Stable-isotope probing (SIP), combined with amplicon sequencing of 16S rRNA, nirS and nirK genes, was used to identify the active denitrifiers. Members of the phylum Betaproteobacteria were the dominant denitrifiers in two of three sites, with different families being observed; members of the genus Vogesella (Neisseriaceae) were key denitrifiers at one site, while the genera Dechloromonas (Rhodocyclaceae) or Comamonas (Comamonadaceae) were the main denitrifiers detected at the other sites.

Item Type: Article
Uncontrolled Keywords: denitrification,groundwater,stable isotope probing,nirs,nirk,betaproteobacteria
Faculty \ School: Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 15 Jan 2018 17:30
Last Modified: 19 Aug 2020 23:59
URI: https://ueaeprints.uea.ac.uk/id/eprint/65936
DOI: 10.1093/femsec/fix181

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