Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus

Qi, Xiaoxuan, Wang, Wenjian, Dong, Haohao, Liang, Yuying, Dong, Changjiang and Ly, Hinh (2017) Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus. In: Hemorrhagic Fever Viruses. Hemorrhagic Fever Viruses . Humana Press, pp. 179-188. ISBN 978-1-4939-6980-7

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Abstract

We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable.

Item Type: Book Section
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 19 Oct 2017 05:08
Last Modified: 22 Apr 2020 11:11
URI: https://ueaeprints.uea.ac.uk/id/eprint/65185
DOI: 10.1007/978-1-4939-6981-4_12

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