Neonatal overfeeding alters hypothalamic microglial profiles and central responses to immune challenge long-term

Ziko, Ilvana, De Luca, Simone N, Dinan, Tara, Barwood, Joanne M, Sominsky, Luba, Cai, Guohui, Kenny, Rachel, Stokes, Leanne ORCID:, Jenkins, Trisha A. and Spencer, Sarah J. (2014) Neonatal overfeeding alters hypothalamic microglial profiles and central responses to immune challenge long-term. Brain, Behavior, and Immunity, 41. pp. 32-43. ISSN 0889-1591

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The early life period is one of significant vulnerability to programming effects from the environment. Given the sensitivity of microglial cells to early life programming and to adult diet, we hypothesized overfeeding during the neonatal period would acutely alter microglial profiles within the developing brain, predisposing the individual to a lasting central pro-inflammatory profile that contributes to overactive immune responses long-term. We tested this idea by manipulating litter sizes in which Wistar rat pups were raised, so the pups were suckled in litters of 4 (neonatally overfed) or 12 (control). This manipulation induces obesity and susceptibility to lipopolysaccharide (LPS) long-term. We then examined microglial and central pro-inflammatory profiles during development and in adulthood as well as susceptibility to neuroimmune challenge with LPS. Neonatally overfed rats have evidence of microgliosis in the paraventricular nucleus of the hypothalamus (PVN) as early as postnatal day 14. They also show changes in hypothalamic gene expression at this time, with suppressed hypothalamic interleukin 1β mRNA. These effects persist into adulthood, with basal PVN microgliosis and increased hypothalamic toll-like receptor 4, nuclear factor κB, and interleukin 6 gene expression. These neonatally overfed rats also have dramatically exacerbated microglial activation in the PVN 24h after an adult LPS challenge, coupled with changes in inflammatory gene expression. Thus, it appears neonatal overfeeding sensitizes PVN microglia, contributing to a basal pro-inflammatory profile and an altered response to a neuroimmune challenge throughout life. It remains to be seen if these effects can be reversed with early interventions.

Item Type: Article
Additional Information: Copyright © 2014 Elsevier Inc. All rights reserved.
Uncontrolled Keywords: ionized calcium-binding adapter molecule-1 (iba-1),interleukin 6 (il-6),lipopolysaccharide (lps),paraventricular nucleus of the hypothalamus (pvn),toll-like receptor 4 (tlr4),sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Science > School of Pharmacy
UEA Research Groups: Faculty of Science > Research Groups > Molecular and Tissue Pharmacology
Depositing User: Pure Connector
Date Deposited: 18 Oct 2017 05:08
Last Modified: 22 Oct 2022 03:16
DOI: 10.1016/j.bbi.2014.06.014

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