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Rusli, Fenni, Boekschoten, Mark V., Borelli, Vincenzo, Sun, Chen, Lute, Carolien, Menke, Aswin L., van den Heuvel, Joost, Salvioli, Stefano, Franceschi, Claudio, Muller, Michael 
ORCID: https://orcid.org/0000-0002-5930-9905 and Steegenga, Wilma T.
(2018)
Plasticity of lifelong calorie-restricted C57BL/6J mice in adapting to a medium-fat diet intervention at old age.
Aging Cell, 17 (2).
ISSN 1474-9718
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Abstract
Calorie restriction (CR) is a dietary regimen that supports healthy aging. In this study we investigated the systemic and liver-specific responses caused by a diet switch to a medium-fat (MF) diet in 24-month-old life-long, CR-exposed mice. This study aimed to increase the knowledge base on dietary alterations of gerontological relevance. Nine-week-old C57BL/6J mice were exposed either to a control, CR or MF diet. At the age of 24 months, a subset of mice of the CR group was transferred to ad libitum MF feeding (CR-MF). The mice were sacrificed at the age of 28 months, then biochemical and molecular analyses were performed. Our results showed that, despite the long-term exposure to the CR regimen, mice in the CR-MF group displayed hyperphagia, rapid weight gain, and hepatic steatosis. However, no hepatic fibrosis/injury or alteration in CR-improved survival was observed in the diet switch group. The liver transcriptomic profile of CR-MF mice largely shifted to a profile similar to the MF-fed animals but leaving ~22% of the 1578 differentially regulated genes between the CR and MF diet groups comparable with the expression of the life-long CR group. Therefore, although the diet switch was performed at an old age, the CR-MF-exposed mice showed plasticity in coping with the challenge of a MF diet without developing severe liver pathologies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | aging,long-term cr,liver,nafld,transcriptomics,glycomics |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 27 Sep 2017 16:14 |
| Last Modified: | 22 Oct 2022 03:12 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/64975 |
| DOI: | 10.1111/acel.12696 |
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