Trypanosoma brucei: trypanocidal and cell swelling activities of lasalocid acid

Steverding, Dietmar and Huczyński, Adam (2017) Trypanosoma brucei: trypanocidal and cell swelling activities of lasalocid acid. Parasitology Research, 116 (11). 3229–3233. ISSN 0932-0113

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Abstract

Chemotherapeutic treatment of human and animal trypanosomaisis is unsatisfactory because only a few drugs are available. As these drugs have poor efficacy and cause adverse reactions, more effective and tolerable medications are needed. As the polyether ionophore antibiotic lasalocid acid is used as medicated feed additive in cattle, the compound was tested for its trypanocidal and cytotoxic activity against bloodstream forms of Trypanosoma brucei and human myeloid HL-60 cells. The concentrations required of lasalocid acid to reduce the growth rate of trypanosomes by 50% and to kill the parasites were 1.75 µM and 10 µM respectively. The ionophore displayed also cytotoxic activity against HL-60 cells but the human cells were about 10 to 14 times less sensitive indicating moderate selectivity. As the trypanocidal mechanism of action of polyether ionophore antibiotics is due to a sodium influx-induced cell swelling, the effect of lasalocid acid on cell volume change in bloodstream-form trypanosomes was investigated. Interestingly, lasalocid acid induced a much faster cell swelling in trypanosomes than the more trypanocidal related ionophore salinomycin. These results support further investigations of lasalocid acid and derivatives thereof as potential agents against African trypanosomiasis.

Item Type: Article
Uncontrolled Keywords: african trypanosomiasis,trypanosoma brucei,lasalocid acid,polyether ionophores
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 19 Sep 2017 05:07
Last Modified: 14 Nov 2020 00:58
URI: https://ueaeprints.uea.ac.uk/id/eprint/64916
DOI: 10.1007/s00436-017-5624-6

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