Photoactivation of the BLUF protein PixD Probed by the Site-Specific Incorporation of Fluorotyrosine Residues

Gil, Agnieszka A., Laptenok, Sergey P., Iuliano, James N., Lukacs, Andras, Verma, Anil, Hall, Christopher R., Yoon, EunBin, Brust, Richard, Greetham, Gregory M., Towrie, Michael, French, Jarrod B., Meech, Stephen R. and Tonge, Peter J. (2017) Photoactivation of the BLUF protein PixD Probed by the Site-Specific Incorporation of Fluorotyrosine Residues. Journal of the American Chemical Society, 139 (41). 14638–14648. ISSN 0002-7863

[img]
Preview
PDF (Accepted manuscript) - Submitted Version
Download (1MB) | Preview

Abstract

The flavin chromophore in blue light using FAD (BLUF) photoreceptors is surrounded by a hydrogen bond network that senses and responds to changes in the electronic structure of the flavin on the ultrafast time scale. The hydrogen bond network includes a strictly conserved Tyr residue, and previously we explored the role of this residue, Y21, in the photoactivation mechanism of the BLUF protein AppA by the introduction of fluorotyrosine (F-Tyr) analogs that modulated the pKa and reduction potential of Y21 by 3.5 pH units and 200 mV, respectively. Although little impact on the forward (dark to light adapted form) photoreaction was observed, the change in Y21 pKa led to a 4,000-fold increase in the rate of dark state recovery. In the present work we have extended these studies to the BLUF protein PixD, where, in contrast to AppA, modulation in the Tyr (Y8) pKa has a profound impact on the forward photoreaction. In particular, a decrease in Y8 pKa by 2 or more pH units prevents formation of a stable light state, consistent with a photoactivation mechanism that involves proton transfer or proton coupled electron transfer from Y8 to the electronically excited FAD. Conversely, the effect of pKa on the rate of dark recovery is markedly reduced in PixD. These observations highlight very significant differences between the photocycles of PixD and AppA, despite their sharing highly conserved FAD binding architectures.

Item Type: Article
Uncontrolled Keywords: pixd,bluf,ultrafast infrared,fluorotyrosine,fad,photoactivation,kinetics
Faculty \ School: Faculty of Science > School of Chemistry
Related URLs:
Depositing User: Pure Connector
Date Deposited: 14 Sep 2017 05:05
Last Modified: 18 Sep 2020 00:14
URI: https://ueaeprints.uea.ac.uk/id/eprint/64872
DOI: 10.1021/jacs.7b07849

Actions (login required)

View Item View Item