A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth

Al-Mamun, Mohammad A, Srisukkham, Worawut, Farid, Dewan Md, Ravenhill, Lorna, Zhang, Li, Hossain, Alamgir and Bass, Rosemary (2018) A quantitative image analysis for the cellular cytoskeleton during in vitro tumor growth. Expert Systems with Applications, 92. 39–51. ISSN 0957-4174

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The cellular cytoskeleton is a dynamic subcellular structure that can be a marker of key biological phenomena including cell division, organelle movement, shape changes and locomotion during the avascular tumor phase. Little attention is paid to quantify changes in the cytoskeleton while nuclei and cytoplasmic both are present in subcellular microscopic images. In this paper, we proposed a quantitative image analysis method to analyze subcellular cytoskeletal changes using a texture analysis method preceded by segmentation of nuclei, cytoplasm and ruffling regions (area except nuclei and cytoplasm). To test and validate this model we hypothesized that Mammary Serine Protease Inhibitor (maspin) acts as cytoskeleton regulator that mediates cell-extracellular matrix (ECM) adhesion in tumor. Maspin-a tumor suppressor gene shows multiple tumor suppressive properties such as increasing tumor cell apoptosis and reducing migration, proliferation, invasion, and overall tumor metastasis. The proposed method obtained separated ruffling regions from segmentation steps and then adopted gray–level histograms (GLH) and grey-level co-occurrence matrix (GLCM) texture analysis techniques. In order to verify the reliability, the proposed texture analysis method was used to compare the control and maspin expressing cells grown on different ECM components: plastic, collagen I, fibronectin and laminin. The results show that the texture parameters extracted reflect the different cytoskeletal changes. These changes indicate that maspin acts as a regulator of the cell-ECM enhancement process, while it reduces the cell migration. Overall, this paper not only presents a quantitative image analysis approach to analyze subcellular cytoskeletal architectures but also provides a comprehensive tool for the biologist, pathologist, cancer specialist, and computer scientist to understand cellular and subcellular organization of cells. In long term, this method can be extended to be used in live cell tracking in in vivo, image informatics based point-of-care expert system and quantification of various complex architectures in organisms.

Item Type: Article
Uncontrolled Keywords: cellular cytoskeleton,maspin,tumor growth,quantitative image analysis,marker-controlled watershed segmentation and texture analysis,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: Pure Connector
Date Deposited: 09 Sep 2017 05:06
Last Modified: 22 Oct 2022 03:09
URI: https://ueaeprints.uea.ac.uk/id/eprint/64801
DOI: 10.1016/j.eswa.2017.08.048


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