The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2)

Som, Nicolle F, Heine, Daniel, Holmes, Neil, Knowles, Felicity, Chandra, Govind, Seipke, Ryan F, Hoskisson, Paul A, Wilkinson, Barrie and Hutchings, Matthew I (2017) The MtrAB two-component system controls antibiotic production in Streptomyces coelicolor A3(2). Microbiology, 163 (10). pp. 1415-1419. ISSN 1350-0872

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Abstract

MtrAB is a highly conserved two component system implicated in the regulation of cell division in the Actinobacteria. It coordinates DNA replication with cell division in the unicellular Mycobacterium tuberculosis and links antibiotic production to sporulation in the filamentous Streptomyces venezuelae. Chloramphenicol biosynthesis is directly regulated by MtrA in S. venezuelae and deletion of mtrB constitutively activates MtrA and results in constitutive over-production of chloramphenicol. Here we report that in Streptomyces coelicolor, MtrA binds to sites upstream of developmental genes and the genes encoding ActII-1, ActII-4 and RedZ, which are cluster situated regulators of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red). Consistent with this, deletion of mtrB switches on production of Act, Red and streptorubin B, a product of the Red pathway. Thus, we propose that MtrA is a key regulator that links antibiotic production to development and it can be used to upregulate antibiotic production in distantly related streptomycetes.

Item Type: Article
Uncontrolled Keywords: sporulation,streptomyces,cryptic gene clusters,antibiotics
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
Depositing User: Pure Connector
Date Deposited: 16 Aug 2017 05:06
Last Modified: 16 Nov 2020 00:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/64514
DOI: 10.1099/mic.0.000524

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