Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b

Gu, Wenyu, Farhan Ul Haque, Muhammad, DiSpirito, Alan A and Semrau, Jeremy D (2016) Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b. FEMS Microbiology Letters, 363 (13). ISSN 0378-1097

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Abstract

It is well known that Methylosinus trichosporium OB3b has two forms of methane monooxygenase (MMO) responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase and a membrane-associated (particulate) methane monooxygenase, and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-type MeDH (Mxa-MeDH) and Xox-type MeDH (Xox-MeDH), and the expression of these two forms is regulated by the availability of the rare earth element (REE), cerium. Here, we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these REEs on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b, where the Mxa-MeDH was knocked out, was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. Collectively, these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b, but that copper overrides the effect of other metals by an as yet unknown mechanism.

Item Type: Article
Uncontrolled Keywords: methanotrophy,methanol dehydrogenase,methane monooxygenase,rare earth elements,copper
Faculty \ School: Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 13 Jul 2017 05:06
Last Modified: 26 Sep 2020 23:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/64106
DOI: 10.1093/femsle/fnw129

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