%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Rosaria Campilongo, Rowena K. Y. Fung, Richard H. Little, Lucia Grenga, Eleftheria Trampari, Simona Pepe, Govind Chandra, Clare E. M. Stevenson, Davide Roncarati, Jacob G. Malone
One ligand, two regulators and three binding sites: How KDPG controls primary carbon metabolism in Pseudomonas
www.plosgenetics.org
www.plosgenetics.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 14 0 R/Contents 15 0 R/TrimBox[0 0 612 792]>>
endobj
14 0 obj
[16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R]
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref001)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref002)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref003)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref004)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref005)>>
endobj
21 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref006)>>
endobj
22 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref007)>>
endobj
23 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref008)>>
endobj
24 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref009)>>
endobj
25 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref010)>>
endobj
26 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref011)>>
endobj
27 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref012)>>
endobj
28 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref014)>>
endobj
29 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref014)>>
endobj
30 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref014)>>
endobj
31 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref015)>>
endobj
32 0 obj
<>/Border[0 0 0]/A 33 0 R>>
endobj
33 0 obj
<>
endobj
15 0 obj
[34 0 R 35 0 R 36 0 R 37 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R 45 0 R 46 0 R 47 0 R 48 0 R 49 0 R]
endobj
34 0 obj
<>stream
q
0.955 g
0 w
199.9998 713.9999 m
575.9997 713.9999 l
575.9997 611.0002 l
199.9998 611.0002 l
f*
0.83 0.64 0.02 0 k
475.0299 523.8992 m
479.7921 523.8992 l
h
f*
278.1921 497.9339 m
282.8976 497.9339 l
h
f*
306.7654 458.9291 m
311.5276 458.9291 l
h
f*
203.4142 432.9071 m
208.1764 432.9071 l
h
f*
212.5984 432.9071 m
217.3606 432.9071 l
h
f*
294.8031 380.9197 m
299.5654 380.9197 l
h
f*
368.9575 367.937 m
373.7197 367.937 l
h
f*
444.3591 341.915 m
449.1213 341.915 l
h
f*
556.4976 328.9323 m
561.2598 328.9323 l
h
f*
470.0409 302.9102 m
479.5654 302.9102 l
h
f*
483.9874 302.9102 m
493.5118 302.9102 l
h
f*
203.4142 263.9055 m
212.9386 263.9055 l
h
f*
218.0976 263.9055 m
227.622 263.9055 l
h
f*
336.8693 250.9228 m
346.3937 250.9228 l
h
f*
466.6394 172.9134 m
476.1071 172.9134 l
h
f*
368.9008 94.9039 m
378.3685 94.9039 l
h
f*
0 g
1 j
1 J
10 0 0 10 209.9905 696.9826 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(gluconeogenesis)Tj
6.718 0 Td
(and)Tj
1.7291 0 Td
(pyruvate)Tj
3.7021 0 Td
(metabolism,)Tj
5.1306 0 Td
(suppressing)Tj
4.9436 0 Td
(the)Tj
1.4684 0 Td
(first)Tj
1.8028 0 Td
(two)Tj
1.7121 0 Td
(pathways)Tj
3.9288 0 Td
(as)Tj
1.0148 0 Td
(pyruvate)Tj
-32.1503 -1.2982 Td
(metabolism)Tj
4.9039 0 Td
(genes)Tj
2.4434 0 Td
(are)Tj
1.4457 0 Td
(expressed,)Tj
4.3199 0 Td
(and)Tj
1.7292 0 Td
(vice)Tj
1.7914 0 Td
(versa.)Tj
2.4888 0 Td
(This)Tj
1.99 0 Td
(complex)Tj
3.6226 0 Td
(regulation)Tj
4.3086 0 Td
(is)Tj
0.8448 0 Td
(enabled)Tj
3.3335 0 Td
(by)Tj
-33.2218 -1.2983 Td
(two)Tj
1.7121 0 Td
(distinct)Tj
3.2088 0 Td
(RccR-binding)Tj
5.7939 0 Td
(consensus)Tj
4.2803 0 Td
(sequences)Tj
4.218 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(RccR)Tj
2.313 0 Td
(regulon)Tj
3.3052 0 Td
(promoters.)Tj
4.6261 0 Td
(KDPG)Tj
-31.9632 -1.3039 Td
(binding)Tj
3.3505 0 Td
(simultaneously)Tj
6.2418 0 Td
(increases)Tj
3.8381 0 Td
(RccR)Tj
2.3074 0 Td
(affinity)Tj
3.0671 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.474 0 Td
(glyoxylate)Tj
4.2122 0 Td
(shunt)Tj
2.4832 0 Td
(and)Tj
1.7291 0 Td
(gluconeogen-)Tj
-30.0867 -1.2983 Td
(esis)Tj
1.6327 0 Td
(promoters,)Tj
4.6204 0 Td
(and)Tj
1.7348 0 Td
(releases)Tj
3.2599 0 Td
(repression)Tj
4.3709 0 Td
(of)Tj
1.0148 0 Td
(pyruvate)Tj
3.7021 0 Td
(metabolism.)Tj
5.1306 0 Td
(This)Tj
1.9843 0 Td
(elegant)Tj
3.0614 0 Td
(two-)Tj
-30.5119 -1.2982 Td
(regulator)Tj
3.8664 0 Td
(circuit)Tj
2.7949 0 Td
(allows)Tj
/F8 1 Tf
2.6986 0 Td
[()18()-3()2()4()4()11()6()11()6( )]TJ
/F7 1 Tf
5.5672 0 Td
(to)Tj
1.0261 0 Td
(rapidly)Tj
3.0388 0 Td
(respond)Tj
3.4752 0 Td
(to)Tj
1.0261 0 Td
(carbon)Tj
3.0104 0 Td
(source)Tj
2.8346 0 Td
(availability)Tj
4.5298 0 Td
(by)Tj
-33.8681 -1.3039 Td
(sensing)Tj
3.1974 0 Td
(a)Tj
0.652 0 Td
(single)Tj
2.5341 0 Td
(key)Tj
1.5931 0 Td
(intermediate,)Tj
5.5502 0 Td
(KDPG.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 568.0062 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 550.9984 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Soil-dwelling)Tj
/F8 1 Tf
5.4765 0 Td
[()18()-3()2()4()4()11()6()11()6( )]TJ
/F7 1 Tf
5.5615 0 Td
(spp.)Tj
1.8425 0 Td
(are)Tj
1.4513 0 Td
(exposed)Tj
3.4413 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.6576 0 Td
(complex)Tj
3.617 0 Td
(and)Tj
1.7348 0 Td
(dynamic)Tj
3.6964 0 Td
(physical)Tj
3.4469 0 Td
(and)Tj
1.7348 0 Td
(chemical)Tj
-33.6867 -1.2982 Td
(environment,)Tj
5.6579 0 Td
(and)Tj
1.7291 0 Td
(must)Tj
2.2223 0 Td
(constantly)Tj
4.3427 0 Td
(adapt)Tj
2.4321 0 Td
(their)Tj
2.109 0 Td
(cell)Tj
1.559 0 Td
(physiology)Tj
4.5241 0 Td
(by)Tj
1.1848 0 Td
(changing)Tj
3.9005 0 Td
(the)Tj
1.4683 0 Td
(expression)Tj
4.4674 0 Td
(pat-)Tj
-35.5972 -1.3039 Td
(terns)Tj
2.2223 0 Td
(of)Tj
1.0148 0 Td
(membrane)Tj
4.5637 0 Td
(proteins,)Tj
3.7418 0 Td
(secreted)Tj
3.4752 0 Td
(small)Tj
2.3244 0 Td
(molecules,)Tj
4.422 0 Td
(and)Tj
1.7348 0 Td
(enzymes)Tj
3.6624 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(].)Tj
1.5987 0 Td
(This)Tj
1.9899 0 Td
(ability)Tj
2.6929 0 Td
(to)Tj
1.0261 0 Td
(gauge)Tj
-34.469 -1.2983 Td
(the)Tj
1.4683 0 Td
(surroundings)Tj
5.6069 0 Td
(and)Tj
1.7291 0 Td
(modulate)Tj
4.0025 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4674 0 Td
(accordingly)Tj
4.8869 0 Td
(is)Tj
0.8504 0 Td
(crucial)Tj
2.9083 0 Td
(for)Tj
1.389 0 Td
(effective)Tj
3.5092 0 Td
(environ-)Tj
-32.8986 -1.2983 Td
(mental)Tj
2.9876 0 Td
(adaptation)Tj
4.4901 0 Td
([)Tj
0.83 0.64 0.02 0 k
(2)Tj
0 g
(].)Tj
1.5987 0 Td
(Therefore,)Tj
4.3937 0 Td
(the)Tj
1.4683 0 Td
(capacity)Tj
3.4696 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()18()-3()2()4()4()11()6()11()6( )]TJ
/F7 1 Tf
5.5672 0 Td
(to)Tj
1.0262 0 Td
(prosper)Tj
3.2881 0 Td
(in)Tj
1.0375 0 Td
(different)Tj
3.6567 0 Td
(niches)Tj
-33.9985 -1.3039 Td
(depends)Tj
3.5489 0 Td
(not)Tj
1.5704 0 Td
(only)Tj
1.9786 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(acquisition)Tj
4.6318 0 Td
(of)Tj
1.0148 0 Td
(nutrients)Tj
3.8607 0 Td
(and)Tj
1.7348 0 Td
(effective)Tj
3.5093 0 Td
(resistance)Tj
4.1272 0 Td
(to)Tj
1.0261 0 Td
(external)Tj
3.4299 0 Td
(stresses,)Tj
-33.1764 -1.2982 Td
(but)Tj
1.5533 0 Td
(also)Tj
1.7688 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(effective)Tj
3.5092 0 Td
(deployment)Tj
4.989 0 Td
(of)Tj
1.0148 0 Td
(sensory)Tj
3.2428 0 Td
(proteins,)Tj
3.7417 0 Td
(signal-transduction)Tj
8.005 0 Td
(pathways)Tj
3.9288 0 Td
(and)Tj
-34.4974 -1.2983 Td
(transcriptional)Tj
6.1114 0 Td
(regulators)Tj
4.2236 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(].)Tj
1.6044 0 Td
(While)Tj
2.6475 0 Td
(much)Tj
2.5229 0 Td
(research)Tj
3.5546 0 Td
(into)Tj
1.8425 0 Td
(niche)Tj
2.4094 0 Td
(colonisation)Tj
5.1364 0 Td
(by)Tj
1.1792 0 Td
(microbes)Tj
3.9004 0 Td
(has)Tj
-35.1323 -1.2983 Td
(focussed)Tj
3.6396 0 Td
(on)Tj
1.2756 0 Td
(phenotypic)Tj
4.7055 0 Td
(adaptations)Tj
4.8472 0 Td
(such)Tj
2.0579 0 Td
(as)Tj
1.0148 0 Td
(motility,)Tj
3.6057 0 Td
(biofilm)Tj
3.1181 0 Td
(formation)Tj
4.2632 0 Td
(and)Tj
1.7292 0 Td
(stress)Tj
2.3981 0 Td
(resistance)Tj
-32.6549 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2585 0 Td
(5)Tj
0 g
(],)Tj
1.2643 0 Td
(the)Tj
1.4683 0 Td
(remodelling)Tj
5.0627 0 Td
(of)Tj
1.0147 0 Td
(central)Tj
2.9707 0 Td
(metabolism)Tj
4.8983 0 Td
(to)Tj
1.0261 0 Td
(optimally)Tj
4.0138 0 Td
(respond)Tj
3.4753 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(external)Tj
3.4299 0 Td
(environment)Tj
-32.3771 -1.2983 Td
(is)Tj
0.8447 0 Td
(a)Tj
0.6576 0 Td
(critical)Tj
2.948 0 Td
(and)Tj
1.7291 0 Td
(understudied)Tj
5.5559 0 Td
(trait.)Tj
-10.5391 -1.2982 Td
(The)Tj
1.7858 0 Td
(efficient)Tj
3.4469 0 Td
(coordination)Tj
5.4255 0 Td
(of)Tj
1.0148 0 Td
(carbon)Tj
3.0103 0 Td
(uptake)Tj
2.9197 0 Td
(and)Tj
1.7291 0 Td
(flux)Tj
1.7462 0 Td
(through)Tj
3.4469 0 Td
(the)Tj
1.4683 0 Td
(primary)Tj
3.4582 0 Td
(metabolic)Tj
4.1443 0 Td
(path-)Tj
-34.7922 -1.3039 Td
(ways)Tj
2.1543 0 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.4684 0 Td
(nutrient)Tj
3.5036 0 Td
(availability)Tj
4.5297 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(surroundings)Tj
5.6012 0 Td
(is)Tj
0.8504 0 Td
(key)Tj
1.5931 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(successful)Tj
4.1612 0 Td
(colonisation)Tj
5.1363 0 Td
(of)Tj
-35.977 -1.2983 Td
(most)Tj
2.2053 0 Td
(ecological)Tj
4.1612 0 Td
(niches)Tj
2.7723 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(],)Tj
1.5987 0 Td
(and)Tj
1.7348 0 Td
(relies)Tj
2.3131 0 Td
(on)Tj
1.2756 0 Td
(multilevel)Tj
4.1725 0 Td
(control)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(expression)Tj
4.4617 0 Td
(and)Tj
1.7291 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
-35.1493 -1.2983 Td
(proteins)Tj
3.5149 0 Td
(involved)Tj
3.6453 0 Td
(in)Tj
1.0375 0 Td
(primary)Tj
3.4582 0 Td
(metabolism)Tj
4.8983 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
1.6044 0 Td
(A)Tj
0.9014 0 Td
(range)Tj
2.4661 0 Td
(of)Tj
1.0148 0 Td
(transcriptional)Tj
6.1115 0 Td
(factors)Tj
2.9026 0 Td
(orchestrates)Tj
-31.555 -1.3039 Td
(bacterial)Tj
3.6226 0 Td
(gene)Tj
2.0806 0 Td
(expression)Tj
4.4674 0 Td
(in)Tj
1.0375 0 Td
(response)Tj
3.7303 0 Td
(to)Tj
1.0262 0 Td
(carbon)Tj
3.0103 0 Td
(source)Tj
2.8347 0 Td
(availability,)Tj
4.7565 0 Td
(generating)Tj
4.4617 0 Td
(a)Tj
0.6576 0 Td
(sophisticated)Tj
-31.6854 -1.2982 Td
(control)Tj
3.118 0 Td
(network)Tj
3.5547 0 Td
(for)Tj
1.3833 0 Td
(both)Tj
2.0579 0 Td
(catabolic)Tj
3.7644 0 Td
(and)Tj
1.7291 0 Td
(anabolic)Tj
3.5886 0 Td
(metabolism)Tj
4.8983 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.6044 0 Td
(Transcription)Tj
5.7429 0 Td
(factors)Tj
2.9027 0 Td
(belong-)Tj
-34.3443 -1.2983 Td
(ing)Tj
1.5023 0 Td
(to)Tj
1.0205 0 Td
(the)Tj
1.474 0 Td
(RpiR)Tj
2.245 0 Td
(family)Tj
2.7326 0 Td
(control)Tj
3.1181 0 Td
(the)Tj
1.4683 0 Td
(expression)Tj
4.4674 0 Td
(of)Tj
1.0148 0 Td
(enzymes)Tj
3.6623 0 Td
(involved)Tj
3.651 0 Td
(in)Tj
1.0375 0 Td
(carbon)Tj
3.0104 0 Td
(metabolism)Tj
4.9039 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
(].)Tj
-35.3081 -1.2983 Td
(Members)Tj
4.0195 0 Td
(of)Tj
1.0147 0 Td
(this)Tj
1.6725 0 Td
(family)Tj
2.7269 0 Td
(are)Tj
1.4513 0 Td
(characterized)Tj
5.5559 0 Td
(by)Tj
1.1849 0 Td
(a)Tj
0.6519 0 Td
(helix-turn-helix)Tj
6.5253 0 Td
(DNA-binding)Tj
5.8621 0 Td
(domain)Tj
3.3221 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
-34.9396 -1.3039 Td
(N-terminus)Tj
4.9209 0 Td
(and)Tj
1.7348 0 Td
(a)Tj
0.6519 0 Td
(sugar)Tj
2.3811 0 Td
(isomerase)Tj
4.1839 0 Td
(domain)Tj
3.3279 0 Td
(\(SIS\))Tj
2.1883 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4684 0 Td
(C-terminus)Tj
4.8529 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(11)Tj
0 g
(].)Tj
1.7404 0 Td
(In)Tj
/F8 1 Tf
1.1055 0 Td
[()18()-3()2()4()4()11()6()11()6( )]TJ
/F7 1 Tf
(s,)Tj
-31.2432 -1.2983 Td
(the)Tj
1.4683 0 Td
(RpiR)Tj
2.245 0 Td
(regulator)Tj
3.8664 0 Td
(HexR)Tj
2.5002 0 Td
(controls)Tj
3.4809 0 Td
(the)Tj
1.4683 0 Td
(uptake)Tj
2.9197 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(catabolism)Tj
4.5184 0 Td
(of)Tj
1.0148 0 Td
(glucose,)Tj
3.4015 0 Td
(modulating)Tj
4.8586 0 Td
(the)Tj
-34.9396 -1.2982 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(glucose)Tj
3.1747 0 Td
(phosphorylative)Tj
6.6387 0 Td
(pathway)Tj
3.566 0 Td
(and)Tj
1.7348 0 Td
(Entner-Doudoroff)Tj
7.5855 0 Td
(\(ED\))Tj
2.2053 0 Td
(pathway)Tj
3.5716 0 Td
(genes)Tj
-33.9531 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
3.5432 0 Td
(HexR)Tj
2.5002 0 Td
(gene)Tj
2.0806 0 Td
(targets)Tj
2.8686 0 Td
(are)Tj
1.4514 0 Td
(grouped)Tj
3.5603 0 Td
(in)Tj
1.0318 0 Td
(two)Tj
1.7121 0 Td
(well-conserved)Tj
6.2135 0 Td
(operons)Tj
3.4526 0 Td
(containing)Tj
4.5297 0 Td
(genes)Tj
2.4378 0 Td
(for)Tj
-35.3818 -1.2983 Td
(glucose)Tj
3.1747 0 Td
(catabolism)Tj
4.5128 0 Td
(and)Tj
1.7348 0 Td
(transport)Tj
3.9231 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
2.0806 0 Td
(HexR)Tj
2.4945 0 Td
(binds)Tj
2.4321 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(dimer)Tj
2.6306 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(pseudo-palindromic)Tj
-27.1443 -1.2983 Td
(DNA)Tj
2.3697 0 Td
(consensus)Tj
4.2803 0 Td
(sequence)Tj
3.8551 0 Td
(5'-)Tj
1.2586 0 Td
(TTGTN7-8ACAA-3',)Tj
8.7873 0 Td
(found)Tj
2.6135 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[(\n)-24()]TJ
/F7 1 Tf
(/)Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
3.8437 0 Td
(and)Tj
/F8 1 Tf
1.7235 0 Td
[()7()4()]TJ
/F7 1 Tf
(/)Tj
/F8 1 Tf
[()2( )12()]TJ
/F7 1 Tf
4.1045 0 Td
(inter-)Tj
-35.3364 -1.3039 Td
(genic)Tj
2.347 0 Td
(regions,)Tj
3.3902 0 Td
(and)Tj
1.7292 0 Td
(represses)Tj
3.8324 0 Td
(gene)Tj
2.0806 0 Td
(transcription)Tj
5.4311 0 Td
(in)Tj
1.0375 0 Td
(its)Tj
1.1452 0 Td
(apo-form.)Tj
4.2406 0 Td
(When)Tj
2.6872 0 Td
(glucose)Tj
3.1748 0 Td
(is)Tj
0.8447 0 Td
(present,)Tj
3.3903 0 Td
(it)Tj
0.788 0 Td
(is)Tj
-36.1188 -1.2983 Td
(imported)Tj
3.9514 0 Td
(and)Tj
1.7291 0 Td
(metabolised)Tj
5.0343 0 Td
(via)Tj
1.3777 0 Td
(several)Tj
2.931 0 Td
(steps)Tj
2.1826 0 Td
(to)Tj
1.0262 0 Td
(2-keto-3-deoxy-6-phospho)Tj
10.7489 0 Td
(gluconate)Tj
4.0932 0 Td
(\(KDPG\),)Tj
-33.0744 -1.2982 Td
(an)Tj
1.2018 0 Td
(exclusive)Tj
3.8098 0 Td
(metabolic)Tj
4.1499 0 Td
(intermediate)Tj
5.3234 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(ED)Tj
1.5251 0 Td
(pathway.)Tj
3.7927 0 Td
(HexR)Tj
2.5001 0 Td
(binds)Tj
2.4321 0 Td
(KDPG)Tj
2.9027 0 Td
(via)Tj
1.3776 0 Td
(its)Tj
1.1452 0 Td
(SIS)Tj
1.5137 0 Td
(domain,)Tj
-34.1572 -1.3039 Td
(signalling)Tj
4.0705 0 Td
(glucose)Tj
3.1691 0 Td
(availability)Tj
4.5297 0 Td
(and)Tj
1.7348 0 Td
(causing)Tj
3.2428 0 Td
(HexR)Tj
2.5002 0 Td
(to)Tj
1.0261 0 Td
(dissociate)Tj
4.0875 0 Td
(from)Tj
2.2054 0 Td
(DNA,)Tj
2.6022 0 Td
(inducing)Tj
3.7984 0 Td
(expression)Tj
-32.9667 -1.2983 Td
(of)Tj
1.0147 0 Td
(its)Tj
1.1452 0 Td
(gene)Tj
2.0807 0 Td
(targets)Tj
2.8743 0 Td
(and)Tj
1.7291 0 Td
(stimulating)Tj
4.7508 0 Td
(glucose)Tj
3.1748 0 Td
(uptake)Tj
2.9197 0 Td
(and)Tj
1.7291 0 Td
(metabolism)Tj
4.9039 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
-0.006 Tc
-25.1261 -1.2983 Td
(Downstream)Tj
5.3858 0 Td
(of)Tj
1.0091 0 Td
(glycolysis,)Tj
4.2122 0 Td
(the)Tj
1.4627 0 Td
(Krebs)Tj
2.5455 0 Td
(cycle)Tj
2.1827 0 Td
(is)Tj
0.8447 0 Td
(responsible)Tj
4.7281 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4627 0 Td
(complete)Tj
3.8608 0 Td
(oxidation)Tj
4.0251 0 Td
(of)Tj
-34.2989 -1.2982 Td
(acetyl-CoA)Tj
4.6828 0 Td
(to)Tj
1.0204 0 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 271.1055 146.3244 cm
BT
/F7 1 Tf
1 TL
-0.008 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 276.8314 148.0251 cm
BT
/F7 1 Tf
1 TL
-0.006 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(provides)Tj
3.634 0 Td
(intermediates)Tj
5.6522 0 Td
(that)Tj
1.7745 0 Td
(are)Tj
1.44 0 Td
(necessary)Tj
4.0025 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.4626 0 Td
(production)Tj
4.6942 0 Td
(of)Tj
1.0091 0 Td
(amino)Tj
-34.4633 -1.3039 Td
(acids)Tj
2.2224 0 Td
(and)Tj
1.7234 0 Td
(other)Tj
2.3357 0 Td
(cellular)Tj
3.1237 0 Td
(macromolecules.)Tj
6.9505 0 Td
(The)Tj
1.7745 0 Td
(pools)Tj
2.3471 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.245 0 Td
(metabolic)Tj
4.1272 0 Td
(intermediates)Tj
5.6579 0 Td
(must)Tj
2.2167 0 Td
(be)Tj
-35.7389 -1.2982 Td
(constantly)Tj
4.32 0 Td
(resupplied)Tj
4.3822 0 Td
(to)Tj
1.0205 0 Td
(maintain)Tj
3.8211 0 Td
(them)Tj
2.279 0 Td
(at)Tj
0.9524 0 Td
(sufficient)Tj
3.8891 0 Td
(levels)Tj
2.3641 0 Td
(for)Tj
1.3833 0 Td
(metabolism)Tj
4.8813 0 Td
(and)Tj
1.7234 0 Td
(growth.)Tj
3.2825 0 Td
(For)Tj
1.6214 0 Td
(this)Tj
-35.9203 -1.2983 Td
(reason)Tj
2.8516 0 Td
(it)Tj
0.7824 0 Td
(is)Tj
0.8447 0 Td
(important)Tj
4.2632 0 Td
(that)Tj
1.7688 0 Td
(flux)Tj
1.7405 0 Td
(through)Tj
3.4299 0 Td
(and)Tj
1.7234 0 Td
(around)Tj
3.1238 0 Td
(the)Tj
1.4627 0 Td
(Krebs)Tj
2.5511 0 Td
(cycle)Tj
2.177 0 Td
(is)Tj
0.8447 0 Td
(exquisitely)Tj
4.4391 0 Td
(controlled)Tj
4.3029 0 Td
(to)Tj
-36.3058 -1.3039 Td
(make)Tj
2.3868 0 Td
(best)Tj
1.7971 0 Td
(use)Tj
1.5307 0 Td
(of)Tj
1.0091 0 Td
(available)Tj
3.6453 0 Td
(carbon)Tj
2.999 0 Td
(sources)Tj
3.1805 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
2.0692 0 Td
(During)Tj
3.1238 0 Td
(Krebs)Tj
2.5455 0 Td
(cycle)Tj
2.1827 0 Td
(operation,)Tj
4.2973 0 Td
(acetyl-CoA)Tj
4.6884 0 Td
(con-)Tj
-35.4554 -1.2983 Td
(denses)Tj
2.8516 0 Td
(with)Tj
1.99 0 Td
(oxaloacetate)Tj
5.0908 0 Td
(to)Tj
1.0205 0 Td
(form)Tj
2.1997 0 Td
(citrate,)Tj
2.9423 0 Td
(which)Tj
2.6419 0 Td
(is,)Tj
1.0658 0 Td
(following)Tj
3.9628 0 Td
(a)Tj
0.652 0 Td
(complete)Tj
3.8608 0 Td
(turn)Tj
1.9615 0 Td
(of)Tj
1.0091 0 Td
(the)Tj
1.4627 0 Td
(cycle,)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 133.6819 23.6976 re
W* n
q
133.6252 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 396.7937 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Transcrip)Tj
4.096 0 Td
(tional)Tj
2.5583 0 Td
(control)Tj
3.1607 0 Td
(of)Tj
/F6 1 Tf
1.0417 0 Td
(Pseudomo)Tj
4.7056 0 Td
(nas)Tj
/F0 1 Tf
1.8071 0 Td
(metabolism)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
95.5276 34.9228 m
251.8299 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Genetics)Tj
4.1102 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4749 0 Td
(https://)Tj
2.9551 0 Td
(doi.org/10.13)Tj
5.7331 0 Td
(71/journal.p)Tj
5.1237 0 Td
(gen.100)Tj
3.5433 0 Td
(6839)Tj
0 g
3.1819 0 Td
(June)Tj
2.3528 0 Td
(28,)Tj
1.5803 0 Td
(2017)Tj
33.2294 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(29)Tj
/F11 1 Tf
-0.025 Tc
-66.402 84.0904 Td
(and)Tj
1.5874 0 Td
(analysis,)Tj
3.4087 0 Td
(decision)Tj
3.2953 0 Td
(to)Tj
0.9496 0 Td
(publish,)Tj
3.1464 0 Td
(or)Tj
0.9993 0 Td
(preparation)Tj
4.4362 0 Td
(of)Tj
-17.8229 -1.3748 Td
(the)Tj
1.3677 0 Td
(manuscript.)Tj
/F10 1 Tf
-1.3677 -2.126 Td
(Competing)Tj
4.3795 0 Td
(interests)Tj
3.3307 0 Td
(:)Tj
/F11 1 Tf
0.4819 0 Td
(The)Tj
1.5875 0 Td
(authors)Tj
3.0401 0 Td
(have)Tj
1.963 0 Td
(declared)Tj
-14.7827 -1.3749 Td
(that)Tj
1.6228 0 Td
(no)Tj
1.1693 0 Td
(competing)Tj
4.1599 0 Td
(interests)Tj
ET
endstream
endobj
35 0 obj
<>stream
endstream
endobj
36 0 obj
<>stream
endstream
endobj
37 0 obj
<>stream
endstream
endobj
38 0 obj
<>stream
endstream
endobj
39 0 obj
<>stream
endstream
endobj
40 0 obj
<>stream
endstream
endobj
41 0 obj
<>stream
endstream
endobj
42 0 obj
<>stream
endstream
endobj
43 0 obj
<>stream
endstream
endobj
44 0 obj
<>stream
endstream
endobj
45 0 obj
<>stream
endstream
endobj
46 0 obj
<>stream
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
BT
10.3394 79.2147 Td
(exist.)Tj
ET
Q
endstream
endobj
50 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 51 0 R/Contents 52 0 R/TrimBox[0 0 612 792]>>
endobj
51 0 obj
[53 0 R 54 0 R 55 0 R 56 0 R 57 0 R 58 0 R 59 0 R 60 0 R 61 0 R 62 0 R 63 0 R 64 0 R 65 0 R 66 0 R 67 0 R 68 0 R 69 0 R 70 0 R 71 0 R 72 0 R]
endobj
53 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref016)>>
endobj
54 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref017)>>
endobj
55 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref018)>>
endobj
56 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref019)>>
endobj
57 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref020)>>
endobj
58 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref018)>>
endobj
59 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref021)>>
endobj
60 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref022)>>
endobj
61 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref024)>>
endobj
62 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref003)>>
endobj
63 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref025)>>
endobj
64 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref026)>>
endobj
65 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref027)>>
endobj
66 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref005)>>
endobj
67 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref028)>>
endobj
68 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref004)>>
endobj
69 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref029)>>
endobj
70 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref030)>>
endobj
71 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref014)>>
endobj
72 0 obj
<>/Border[0 0 0]/A 73 0 R>>
endobj
73 0 obj
<>
endobj
52 0 obj
[74 0 R 75 0 R 76 0 R 77 0 R 78 0 R 79 0 R 80 0 R 81 0 R 82 0 R 83 0 R 84 0 R 85 0 R 86 0 R 87 0 R 88 0 R 89 0 R 90 0 R 91 0 R 92 0 R 93 0 R 94 0 R]
endobj
74 0 obj
<>stream
q
0.83 0.64 0.02 0 k
436.5354 667.3323 m
445.9465 667.3323 l
h
f*
450.3685 667.3323 m
459.7795 667.3323 l
h
f*
360.737 654.3496 m
370.148 654.3496 l
h
f*
308.5795 641.3102 m
317.9906 641.3102 l
h
f*
322.4126 641.3102 m
331.8236 641.3102 l
h
f*
526.5071 641.3102 m
535.9181 641.3102 l
h
f*
540.2835 641.3102 m
549.6945 641.3102 l
h
f*
258.9732 563.3008 m
268.3843 563.3008 l
h
f*
273.5433 563.3008 m
282.9543 563.3008 l
h
f*
277.115 511.3134 m
281.8772 511.3134 l
h
f*
269.6315 472.3087 m
279.1559 472.3087 l
h
f*
283.6346 472.3087 m
293.1591 472.3087 l
h
f*
203.4142 459.326 m
212.9386 459.326 l
h
f*
453.8835 446.3433 m
458.6457 446.3433 l
h
f*
463.1244 446.3433 m
472.6488 446.3433 l
h
f*
342.8787 433.3039 m
347.6409 433.3039 l
h
f*
352.1197 433.3039 m
361.6441 433.3039 l
h
f*
455.0173 407.3386 m
464.485 407.3386 l
h
f*
222.5197 303.3071 m
232.0441 303.3071 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.009 Tc
0 0 Td
(reconverted)Tj
4.9379 0 Td
(to)Tj
1.0148 0 Td
(oxaloacetate.)Tj
5.2724 0 Td
(Each)Tj
2.1543 0 Td
(turn)Tj
1.9445 0 Td
(of)Tj
1.0092 0 Td
(this)Tj
1.6497 0 Td
(cycle)Tj
2.1714 0 Td
(involves)Tj
3.4412 0 Td
(the)Tj
1.4513 0 Td
(loss)Tj
1.6781 0 Td
(of)Tj
1.0035 0 Td
(two)Tj
1.6951 0 Td
(molecules)Tj
4.1556 0 Td
(of)Tj
1.0034 0 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 559.8425 705.7133 cm
BT
/F7 1 Tf
1 TL
-0.012 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 563.3574 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.009 Tc
0 0 Td
(.)Tj
-36.3342 -1.3039 Td
(When)Tj
2.6645 0 Td
(acetyl-CoA)Tj
4.6545 0 Td
(is)Tj
0.839 0 Td
(the)Tj
1.4514 0 Td
(only)Tj
1.9559 0 Td
(available)Tj
3.6226 0 Td
(carbon)Tj
2.9821 0 Td
(source,)Tj
3.0273 0 Td
(classical)Tj
3.3676 0 Td
(Krebs)Tj
2.5341 0 Td
(cycle)Tj
2.1657 0 Td
(operation)Tj
4.0478 0 Td
(cannot)Tj
-33.3125 -1.2982 Td
(assimilate)Tj
4.0819 0 Td
(carbon.)Tj
3.1974 0 Td
(Consequently,)Tj
5.8904 0 Td
(when)Tj
2.3867 0 Td
(acetate)Tj
2.9197 0 Td
(or)Tj
1.0885 0 Td
(fatty)Tj
1.9672 0 Td
(acids)Tj
2.2054 0 Td
(are)Tj
1.4343 0 Td
(the)Tj
1.457 0 Td
(primary)Tj
3.4185 0 Td
(source)Tj
2.8063 0 Td
(of)Tj
1.0035 0 Td
(carbon)Tj
-33.8568 -1.2983 Td
(and)Tj
1.7121 0 Td
(energy,)Tj
3.1011 0 Td
(many)Tj
2.4548 0 Td
(bacterial)Tj
3.5773 0 Td
(species)Tj
2.965 0 Td
(including)Tj
/F8 1 Tf
3.9968 0 Td
[()18()-3()2()4()4()11()6()11()6( )]TJ
/F7 1 Tf
5.5105 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7178 0 Td
(17)Tj
0 g
(])Tj
1.4967 0 Td
(activate)Tj
3.2088 0 Td
(a)Tj
0.652 0 Td
(specific)Tj
3.1521 0 Td
(anaplero-)Tj
-33.545 -1.2983 Td
(tic)Tj
1.1849 0 Td
(pathway,)Tj
3.753 0 Td
(called)Tj
2.4831 0 Td
(the)Tj
1.4514 0 Td
(Glyoxylate)Tj
4.4107 0 Td
(shunt)Tj
2.4547 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
2.041 0 Td
(The)Tj
1.7631 0 Td
(Glyoxylate)Tj
4.4107 0 Td
(shunt)Tj
2.4548 0 Td
(is)Tj
0.8334 0 Td
(common)Tj
3.8097 0 Td
(among)Tj
2.9594 0 Td
(microor-)Tj
-34.0099 -1.3039 Td
(ganisms)Tj
3.4412 0 Td
(and)Tj
1.7121 0 Td
(higher)Tj
2.7723 0 Td
(plants)Tj
2.5965 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7178 0 Td
(20)Tj
0 g
(],)Tj
1.7178 0 Td
(and)Tj
1.7121 0 Td
(diverts)Tj
2.8913 0 Td
(part)Tj
1.8199 0 Td
(of)Tj
1.0034 0 Td
(the)Tj
1.457 0 Td
(carbon)Tj
2.9821 0 Td
(flux)Tj
1.7234 0 Td
(at)Tj
0.9411 0 Td
(isocitrate)Tj
3.8211 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7178 0 Td
(21)Tj
0 g
(].)Tj
1.7177 0 Td
(The)Tj
-35.7446 -1.2982 Td
(key)Tj
1.576 0 Td
(enzymes)Tj
3.634 0 Td
(of)Tj
1.0035 0 Td
(this)Tj
1.6554 0 Td
(pathway)Tj
3.532 0 Td
(are)Tj
1.4343 0 Td
(isocitrate)Tj
3.8211 0 Td
(lyase)Tj
2.1146 0 Td
(\(ICL\),)Tj
2.6362 0 Td
(which)Tj
2.6249 0 Td
(converts)Tj
3.5659 0 Td
(isocitrate)Tj
3.8211 0 Td
(to)Tj
1.0148 0 Td
(glyoxylate,)Tj
-32.4338 -1.2983 Td
(and)Tj
1.7121 0 Td
(malate)Tj
2.846 0 Td
(dehydrogenase,)Tj
6.3722 0 Td
(which)Tj
2.6249 0 Td
(condenses)Tj
4.2973 0 Td
(a)Tj
0.6519 0 Td
(molecule)Tj
3.7984 0 Td
(of)Tj
1.0035 0 Td
(glyoxylate)Tj
4.1612 0 Td
(with)Tj
1.9786 0 Td
(an)Tj
1.1962 0 Td
(acetyl-CoA)Tj
4.6545 0 Td
(to)Tj
-35.2968 -1.3039 Td
(produce)Tj
3.4809 0 Td
(malate)Tj
2.846 0 Td
(and)Tj
1.7178 0 Td
(succinate.)Tj
4.0988 0 Td
(This)Tj
1.9673 0 Td
(ensures)Tj
3.2031 0 Td
(the)Tj
1.4513 0 Td
(replenishment)Tj
5.9357 0 Td
(of)Tj
1.0035 0 Td
(the)Tj
1.4513 0 Td
(metabolic)Tj
4.1046 0 Td
(intermediates)Tj
-31.2603 -1.2983 Td
(required)Tj
3.6 0 Td
(for)Tj
1.3663 0 Td
(the)Tj
1.457 0 Td
(biosynthesis)Tj
5.0456 0 Td
(of)Tj
1.0035 0 Td
(cellular)Tj
3.0954 0 Td
(components.)Tj
5.3461 0 Td
(The)Tj
1.7688 0 Td
(only)Tj
1.9559 0 Td
(regulatory)Tj
4.2689 0 Td
(mechanism)Tj
4.8019 0 Td
(for)Tj
1.3663 0 Td
(the)Tj
-35.0757 -1.2983 Td
(Glyoxylate)Tj
4.4163 0 Td
(shunt)Tj
2.4548 0 Td
(known)Tj
2.9821 0 Td
(to)Tj
1.0148 0 Td
(date)Tj
1.8821 0 Td
(involves)Tj
3.4413 0 Td
(the)Tj
1.457 0 Td
(reversible)Tj
4.0195 0 Td
(phosphorylation)Tj
6.7351 0 Td
(of)Tj
1.0034 0 Td
(isocitrate)Tj
3.8211 0 Td
(dehydro-)Tj
-33.2275 -1.3039 Td
(genase)Tj
2.846 0 Td
(\(IDH\))Tj
2.7155 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
3.5093 0 Td
(When)Tj
2.6645 0 Td
(bacteria)Tj
3.3392 0 Td
(grow)Tj
2.2281 0 Td
(on)Tj
1.2642 0 Td
(two-carbon)Tj
4.8019 0 Td
(compounds,)Tj
5.1306 0 Td
(such)Tj
2.041 0 Td
(as)Tj
1.0034 0 Td
(acetate,)Tj
3.1408 0 Td
(IDH)Tj
-34.6845 -1.2982 Td
(phosphorylation)Tj
6.7351 0 Td
(inactivates)Tj
4.3766 0 Td
(this)Tj
1.6555 0 Td
(enzyme)Tj
3.2711 0 Td
(and)Tj
1.7121 0 Td
(forces)Tj
2.5682 0 Td
(carbon)Tj
2.9764 0 Td
(flux)Tj
1.7291 0 Td
(through)Tj
3.4129 0 Td
(the)Tj
1.4513 0 Td
(shunt)Tj
2.4605 0 Td
(pathway.)Tj
/F8 1 Tf
-0.004 Tc
-31.1526 -1.2983 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9978 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
4.4163 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.652 0 Td
(common)Tj
3.8494 0 Td
(soil)Tj
1.5988 0 Td
(bacteria)Tj
3.3788 0 Td
(that)Tj
1.7802 0 Td
(non-specifically)Tj
6.5253 0 Td
(colonizes)Tj
3.9174 0 Td
(plant)Tj
2.2621 0 Td
(roots,)Tj
2.4888 0 Td
(and)Tj
1.7291 0 Td
(can)Tj
-35.6426 -1.3039 Td
(improve)Tj
3.5773 0 Td
(plant)Tj
2.2677 0 Td
(health)Tj
2.6759 0 Td
(through)Tj
3.4469 0 Td
(nutrient)Tj
3.5036 0 Td
(recycling,)Tj
4.0705 0 Td
(pathogen)Tj
3.9401 0 Td
(antagonism,)Tj
5.1251 0 Td
(and)Tj
1.7347 0 Td
(inducing)Tj
3.7928 0 Td
(plant)Tj
-34.1346 -1.2983 Td
(defence)Tj
3.2768 0 Td
(responses)Tj
4.0932 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(].)Tj
1.6044 0 Td
(The)Tj
1.7802 0 Td
(rhizosphere)Tj
4.9322 0 Td
(environment)Tj
5.4312 0 Td
(is)Tj
0.8447 0 Td
(both)Tj
2.0636 0 Td
(highly)Tj
2.7042 0 Td
(complex)Tj
3.6227 0 Td
(and)Tj
1.7348 0 Td
(attractive)Tj
3.9231 0 Td
(to)Tj
-36.0111 -1.2982 Td
(microbial)Tj
4.0592 0 Td
(life.)Tj
1.6724 0 Td
(Plant)Tj
2.3074 0 Td
(roots)Tj
2.262 0 Td
(continuously)Tj
5.4368 0 Td
(produce)Tj
3.5207 0 Td
(and)Tj
1.7291 0 Td
(secrete)Tj
2.9423 0 Td
(compounds)Tj
4.9606 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(rhizosphere)Tj
-31.3963 -1.2983 Td
(environment)Tj
5.4311 0 Td
(\(including)Tj
4.3824 0 Td
(ions,)Tj
2.1259 0 Td
(amino)Tj
2.795 0 Td
(acids,)Tj
2.4604 0 Td
(organic)Tj
3.2372 0 Td
(acids,)Tj
2.4604 0 Td
(sugars,)Tj
2.9651 0 Td
(phenolics)Tj
4.0421 0 Td
(and)Tj
1.7292 0 Td
(other)Tj
2.347 0 Td
(second-)Tj
-33.9758 -1.3039 Td
(ary)Tj
1.4797 0 Td
(metabolites\))Tj
5.142 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7404 0 Td
(26)Tj
0 g
(],)Tj
1.7348 0 Td
(and)Tj
1.7292 0 Td
(many)Tj
2.4774 0 Td
(microorganisms)Tj
6.7464 0 Td
(are)Tj
1.4457 0 Td
(attracted)Tj
3.7304 0 Td
(to)Tj
1.0261 0 Td
(these)Tj
2.2507 0 Td
(exuded)Tj
3.1181 0 Td
(nutrients)Tj
-32.6209 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.0749 0 Td
(Successful)Tj
4.2803 0 Td
(root)Tj
1.8992 0 Td
(colonisation)Tj
5.1364 0 Td
(depends)Tj
3.5489 0 Td
(on)Tj
1.2756 0 Td
(both)Tj
2.0636 0 Td
(the)Tj
1.4684 0 Td
(coordinated)Tj
5.0569 0 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(factors)Tj
-32.2807 -1.2983 Td
(involved)Tj
3.651 0 Td
(in)Tj
1.0318 0 Td
(phenotypes)Tj
4.8019 0 Td
(such)Tj
2.0579 0 Td
(as)Tj
1.0148 0 Td
(motility)Tj
3.3789 0 Td
(and)Tj
1.7291 0 Td
(biofilm)Tj
3.1238 0 Td
(formation)Tj
4.2576 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2642 0 Td
(28)Tj
0 g
(])Tj
1.508 0 Td
(and)Tj
1.7348 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(adaptive)Tj
-32.2977 -1.3039 Td
(remodelling)Tj
5.0626 0 Td
(of)Tj
1.0148 0 Td
(central)Tj
2.9651 0 Td
(metabolism)Tj
4.9039 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2642 0 Td
(29)Tj
0 g
(].)Tj
1.7348 0 Td
(Recently,)Tj
3.8834 0 Td
(an)Tj
1.2076 0 Td
(uncharacterised)Tj
6.582 0 Td
(member)Tj
3.583 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(RpiR)Tj
-34.6845 -1.2982 Td
(family;)Tj
/F8 1 Tf
2.9537 0 Td
[()20()7()]TJ
/F7 1 Tf
1.9162 0 Td
(\()Tj
/F8 1 Tf
[()14()40()]TJ
/F7 1 Tf
(\),)Tj
5.2724 0 Td
(was)Tj
1.7008 0 Td
(identified)Tj
4.0592 0 Td
(in)Tj
1.0318 0 Td
(an)Tj
/F8 1 Tf
1.2018 0 Td
[()39()]TJ
1.0772 0 Td
[()53( )15(!)3()]TJ
2.0523 0 Td
[(")-16()11()20()8()10()10( )4()11()]TJ
4.3823 0 Td
[(#)97()7()13(\r)18()6()10()4()2()-21($)]TJ
/F7 1 Tf
4.6204 0 Td
(\(IVET\))Tj
3.1181 0 Td
(screening)Tj
-33.3862 -1.2983 Td
(experiment)Tj
4.7792 0 Td
(for)Tj
1.3833 0 Td
(loci)Tj
1.6667 0 Td
(involved)Tj
3.6454 0 Td
(in)Tj
/F8 1 Tf
1.0374 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9922 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
4.422 0 Td
(plant)Tj
2.262 0 Td
(interactions)Tj
4.9719 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(].)Tj
2.075 0 Td
(RccR)Tj
2.3074 0 Td
(shares)Tj
2.6985 0 Td
(a)Tj
0.6577 0 Td
(very)Tj
1.9219 0 Td
(high)Tj
-34.8206 -1.3039 Td
(amino-acid)Tj
4.7962 0 Td
(sequence)Tj
3.8607 0 Td
(identity)Tj
3.3052 0 Td
(\(43%)Tj
2.2677 0 Td
(identical\))Tj
3.9968 0 Td
(with)Tj
1.9956 0 Td
(HexR,)Tj
2.7269 0 Td
(and)Tj
1.7292 0 Td
(both)Tj
2.0579 0 Td
(transcription)Tj
5.4311 0 Td
(factors)Tj
2.9084 0 Td
(play)Tj
-35.0757 -1.2983 Td
(important)Tj
4.2803 0 Td
(roles)Tj
2.1259 0 Td
(in)Tj
1.0319 0 Td
(wheat)Tj
2.5908 0 Td
(rhizosphere)Tj
4.9266 0 Td
(colonisation)Tj
5.1363 0 Td
(and)Tj
1.7235 0 Td
(growth)Tj
3.0727 0 Td
(on)Tj
1.2756 0 Td
(defined)Tj
3.2258 0 Td
(carbon)Tj
3.0104 0 Td
(sources.)Tj
3.4185 0 Td
(Fur-)Tj
-35.8183 -1.2983 Td
(thermore,)Tj
4.1839 0 Td
(the)Tj
1.474 0 Td
(HexR/RccR)Tj
4.9153 0 Td
(regulon)Tj
3.3108 0 Td
(is)Tj
0.8447 0 Td
(widespread)Tj
4.7622 0 Td
(among)Tj
2.9934 0 Td
(the)Tj
1.4683 0 Td
(pseudomonads)Tj
6.2815 0 Td
(and)Tj
1.7292 0 Td
(in)Tj
1.0374 0 Td
(several)Tj
-33.0007 -1.2982 Td
(other)Tj
2.3471 0 Td
(bacterial)Tj
3.6226 0 Td
(genera.)Tj
3.1124 0 Td
(This)Tj
1.9899 0 Td
(prompted)Tj
4.201 0 Td
(us)Tj
1.1055 0 Td
(to)Tj
1.0261 0 Td
(investigate)Tj
4.4617 0 Td
(the)Tj
1.4683 0 Td
(contributions)Tj
5.6523 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2564 0 Td
(`twin')Tj
2.4604 0 Td
(tran-)Tj
-34.7185 -1.3039 Td
(scription)Tj
3.7814 0 Td
(factors)Tj
2.9026 0 Td
(to)Tj
1.0262 0 Td
(environmental)Tj
6.1114 0 Td
(adaption)Tj
3.7531 0 Td
(in)Tj
1.0318 0 Td
(more)Tj
2.3414 0 Td
(detail.)Tj
-19.7517 -1.2983 Td
(We)Tj
1.6101 0 Td
(first)Tj
1.8085 0 Td
(examined)Tj
4.1272 0 Td
(the)Tj
1.4683 0 Td
(role)Tj
1.7688 0 Td
(of)Tj
1.0148 0 Td
(HexR)Tj
2.5002 0 Td
(in)Tj
/F8 1 Tf
1.0374 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9922 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
4.4163 0 Td
(during)Tj
2.931 0 Td
(the)Tj
1.4684 0 Td
(wheat)Tj
2.5851 0 Td
(rhizosphere)Tj
4.9323 0 Td
(colonisa-)Tj
-33.8568 -1.2983 Td
(tion,)Tj
2.0636 0 Td
(confirming)Tj
4.7282 0 Td
(its)Tj
1.1451 0 Td
(function)Tj
3.6284 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6576 0 Td
(regulator)Tj
3.8608 0 Td
(of)Tj
1.0148 0 Td
(glucose)Tj
3.1747 0 Td
(metabolism,)Tj
5.1307 0 Td
(consistent)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(earlier)Tj
-32.6719 -1.3039 Td
(data)Tj
1.9105 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
2.0806 0 Td
(Next,)Tj
2.3698 0 Td
(we)Tj
1.3266 0 Td
(characterized)Tj
5.5559 0 Td
(RccR,)Tj
2.5341 0 Td
(and)Tj
1.7348 0 Td
(identified)Tj
4.0592 0 Td
(it)Tj
0.7824 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(master)Tj
2.931 0 Td
(regulator)Tj
3.8665 0 Td
(of)Tj
1.0148 0 Td
(pyruvate)Tj
-31.8329 -1.2982 Td
(metabolism,)Tj
5.125 0 Td
(the)Tj
1.474 0 Td
(glyoxylate)Tj
4.2123 0 Td
(shunt)Tj
2.4831 0 Td
(and)Tj
1.7291 0 Td
(gluconeogenesis.)Tj
6.9449 0 Td
(RccR)Tj
2.313 0 Td
(binds)Tj
2.4321 0 Td
(to)Tj
1.0205 0 Td
(two)Tj
1.7121 0 Td
(distinct,)Tj
3.4356 0 Td
(pseudo-)Tj
-32.8817 -1.2983 Td
(palindromic)Tj
5.1477 0 Td
(binding)Tj
3.3505 0 Td
(sites)Tj
1.9332 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(promoter)Tj
4.0309 0 Td
(regions)Tj
3.1691 0 Td
(of)Tj
1.0148 0 Td
(its)Tj
1.1452 0 Td
(target)Tj
2.5115 0 Td
(genes,)Tj
2.6645 0 Td
(which)Tj
2.6532 0 Td
(share)Tj
2.3357 0 Td
(a)Tj
0.652 0 Td
(binding)Tj
-33.1141 -1.3039 Td
(sequence)Tj
3.8551 0 Td
(but)Tj
1.5534 0 Td
(differ)Tj
2.3867 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(length)Tj
2.7269 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(linker)Tj
2.5739 0 Td
(region.)Tj
3.0274 0 Td
(RccR)Tj
2.3074 0 Td
(shares)Tj
2.6985 0 Td
(a)Tj
0.652 0 Td
(ligand;)Tj
2.9367 0 Td
(the)Tj
1.4683 0 Td
(ED)Tj
1.525 0 Td
(pathway)Tj
-32.7059 -1.2983 Td
(intermediate)Tj
5.3234 0 Td
(2-dehydro-3-deoxy-phosphog)Tj
12.0018 0 Td
(luconate)Tj
3.6284 0 Td
(\(KDPG\),)Tj
3.8097 0 Td
(with)Tj
1.9956 0 Td
(HexR,)Tj
2.7212 0 Td
(reinforcing)Tj
4.7055 0 Td
(the)Tj
1.4683 0 Td
(reg-)Tj
-35.6539 -1.2983 Td
(ulatory)Tj
3.0557 0 Td
(connection)Tj
4.7168 0 Td
(between)Tj
3.532 0 Td
(the)Tj
1.4683 0 Td
(two)Tj
1.7065 0 Td
(proteins.)Tj
3.7417 0 Td
(Furthermore,)Tj
5.6125 0 Td
(ligand)Tj
2.7099 0 Td
(binding)Tj
3.3506 0 Td
(increases)Tj
3.8324 0 Td
(RccR)Tj
-33.7264 -1.3039 Td
(affinity)Tj
3.0671 0 Td
(for)Tj
1.3833 0 Td
(one)Tj
1.7007 0 Td
(binding)Tj
3.3506 0 Td
(site)Tj
1.5703 0 Td
(but)Tj
1.5534 0 Td
(decreases)Tj
3.9685 0 Td
(it)Tj
0.7824 0 Td
(for)Tj
1.3889 0 Td
(the)Tj
1.4684 0 Td
(other,)Tj
2.5738 0 Td
(enabling)Tj
3.6623 0 Td
(a)Tj
0.652 0 Td
(sophisticated)Tj
5.4312 0 Td
(transcrip-)Tj
-32.5529 -1.2982 Td
(tional)Tj
2.5171 0 Td
(response)Tj
3.7361 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.652 0 Td
(single)Tj
2.5341 0 Td
(metabolic)Tj
4.1443 0 Td
(intermediate.)Tj
5.5502 0 Td
(We)Tj
1.6044 0 Td
(propose)Tj
3.4242 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
1.4684 0 Td
(coordinated)Tj
5.057 0 Td
(activity)Tj
3.1179 0 Td
(of)Tj
-36.6119 -1.2983 Td
(HexR)Tj
2.4945 0 Td
(and)Tj
1.7348 0 Td
(RccR)Tj
2.3073 0 Td
(tightly)Tj
2.778 0 Td
(controls)Tj
3.4809 0 Td
(the)Tj
1.474 0 Td
(remodelling)Tj
5.0627 0 Td
(of)Tj
1.0147 0 Td
(central)Tj
2.9651 0 Td
(metabolism)Tj
4.9039 0 Td
(in)Tj
1.0374 0 Td
(response)Tj
3.7304 0 Td
(to)Tj
1.0262 0 Td
(intracel-)Tj
-34.0099 -1.2983 Td
(lular)Tj
2.0466 0 Td
(KDPG)Tj
2.9027 0 Td
(levels,)Tj
2.6021 0 Td
(maximizing)Tj
4.9833 0 Td
(bacterial)Tj
3.6283 0 Td
(proliferation)Tj
5.2668 0 Td
(and)Tj
1.7291 0 Td
(fitness,)Tj
2.9934 0 Td
(and)Tj
1.7291 0 Td
(optimising)Tj
4.5354 0 Td
(enzyme)Tj
3.3052 0 Td
(syn-)Tj
-35.722 -1.3039 Td
(thesis)Tj
2.4548 0 Td
(to)Tj
1.0261 0 Td
(most)Tj
2.2054 0 Td
(effectively)Tj
4.2122 0 Td
(respond)Tj
3.4753 0 Td
(to)Tj
1.0261 0 Td
(nutrient)Tj
3.5036 0 Td
(availability)Tj
4.5297 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(environment.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 145.0204 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.5023 Td
(RccR)Tj
2.6409 0 Td
(and)Tj
1.8662 0 Td
(HexR)Tj
2.6929 0 Td
(are)Tj
1.6441 0 Td
(highly)Tj
2.7922 0 Td
(similar)Tj
3.0709 0 Td
(proteins)Tj
3.7323 0 Td
(and)Tj
1.8661 0 Td
(are)Tj
1.6441 0 Td
(both)Tj
2.1402 0 Td
(important)Tj
4.3465 0 Td
(for)Tj
-28.4364 -1.167 Td
(plant)Tj
2.3574 0 Td
(colonisation)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 95.9811 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
/F8 1 Tf
1.7801 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9978 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
4.4163 0 Td
(SBW25)Tj
/F8 1 Tf
3.1918 0 Td
[()20()7()]TJ
/F7 1 Tf
1.9162 0 Td
(\()Tj
/F8 1 Tf
[()14()40()]TJ
/F7 1 Tf
(\))Tj
5.0457 0 Td
(gene)Tj
2.0806 0 Td
(encodes)Tj
3.4356 0 Td
(an)Tj
1.2018 0 Td
(uncharacterised)Tj
6.5877 0 Td
(member)Tj
3.583 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
-35.2514 -1.2982 Td
(RpiR)Tj
2.245 0 Td
(family)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(transcriptional)Tj
6.1114 0 Td
(regulators.)Tj
4.4504 0 Td
(Following)Tj
4.2179 0 Td
(the)Tj
1.474 0 Td
(observation)Tj
4.8982 0 Td
(that)Tj
/F8 1 Tf
1.7745 0 Td
[()20()7()]TJ
/F7 1 Tf
1.9162 0 Td
(seems)Tj
2.6079 0 Td
(to)Tj
1.0261 0 Td
(play)Tj
1.8709 0 Td
(a)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 133.6819 23.6976 re
W* n
q
133.6252 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 396.7937 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Transcrip)Tj
4.096 0 Td
(tional)Tj
2.5583 0 Td
(control)Tj
3.1607 0 Td
(of)Tj
/F6 1 Tf
1.0417 0 Td
(Pseudomo)Tj
4.7056 0 Td
(nas)Tj
/F0 1 Tf
1.8071 0 Td
(metabolism)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
95.5276 34.9228 m
251.8299 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Genetics)Tj
4.1102 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4749 0 Td
(https://)Tj
2.9551 0 Td
(doi.org/10.13)Tj
5.7331 0 Td
(71/journal.p)Tj
5.1237 0 Td
(gen.100)Tj
3.5433 0 Td
(6839)Tj
0 g
3.1819 0 Td
(June)Tj
2.3528 0 Td
(28,)Tj
1.5803 0 Td
(2017)Tj
33.2294 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
75 0 obj
<>stream
endstream
endobj
76 0 obj
<>stream
endstream
endobj
77 0 obj
<>stream
endstream
endobj
78 0 obj
<>stream
endstream
endobj
79 0 obj
<>stream
endstream
endobj
80 0 obj
<>stream
endstream
endobj
81 0 obj
<>stream
endstream
endobj
82 0 obj
<>stream
endstream
endobj
83 0 obj
<>stream
endstream
endobj
84 0 obj
<>stream
endstream
endobj
85 0 obj
<>stream
endstream
endobj
86 0 obj
<>stream
endstream
endobj
87 0 obj
<>stream
endstream
endobj
88 0 obj
<>stream
endstream
endobj
89 0 obj
<>stream
endstream
endobj
90 0 obj
<>stream
endstream
endobj
91 0 obj
<>stream
endstream
endobj
92 0 obj
<>stream
endstream
endobj
93 0 obj
<>stream
endstream
endobj
94 0 obj
<>stream
BT
66.402 0 Td
(29)Tj
ET
Q
endstream
endobj
95 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 98 0 R/Contents 99 0 R/TrimBox[0 0 612 792]>>
endobj
98 0 obj
[100 0 R 101 0 R 102 0 R 103 0 R 104 0 R 105 0 R 106 0 R 107 0 R 108 0 R 109 0 R 110 0 R 111 0 R 112 0 R 113 0 R 114 0 R 115 0 R]
endobj
100 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref030)>>
endobj
101 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g001)>>
endobj
102 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g001)>>
endobj
103 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref030)>>
endobj
104 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g001)>>
endobj
105 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g002)>>
endobj
106 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g002)>>
endobj
107 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g002)>>
endobj
108 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g002)>>
endobj
109 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g002)>>
endobj
110 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref014)>>
endobj
111 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.ref031)>>
endobj
112 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g003)>>
endobj
113 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g003)>>
endobj
114 0 obj
<>/Border[0 0 0]/Dest(Rpgen.1006839.g003)>>
endobj
115 0 obj
<>/Border[0 0 0]/A 116 0 R>>
endobj
116 0 obj
<>
endobj
99 0 obj
[117 0 R 118 0 R 119 0 R 120 0 R 121 0 R 122 0 R 123 0 R 124 0 R 125 0 R]
endobj
117 0 obj
<>stream
q
0.83 0.64 0.02 0 k
323.0929 706.337 m
332.6173 706.337 l
h
f*
365.1591 654.3496 m
391.578 654.3496 l
h
f*
275.4709 602.3055 m
300.8126 602.3055 l
h
f*
276.4346 576.3402 m
285.9591 576.3402 l
h
f*
467.6598 511.3134 m
493.852 511.3134 l
h
f*
203.4142 387.0992 m
243.6661 387.0992 l
h
f*
498.1606 374.1165 m
539.7165 374.1165 l
h
f*
224.9008 348.0945 m
296.0504 348.0945 l
h
f*
433.7575 322.1291 m
453.3165 322.1291 l
h
f*
424.2331 296.1071 m
449.0646 296.1071 l
h
f*
479.3386 211.9181 m
488.863 211.9181 l
h
f*
493.3417 211.9181 m
502.8661 211.9181 l
h
f*
250.2425 185.8961 m
276.6047 185.8961 l
h
f*
438.2929 185.8961 m
463.6913 185.8961 l
h
f*
370.3748 94.9039 m
396.5669 94.9039 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(role)Tj
1.7688 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(plant)Tj
2.262 0 Td
(environment)Tj
5.4311 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(])Tj
1.8539 0 Td
(we)Tj
1.3266 0 Td
(decided)Tj
3.3392 0 Td
(to)Tj
1.0261 0 Td
(examine)Tj
3.6 0 Td
(the)Tj
1.4683 0 Td
(locus)Tj
2.2848 0 Td
(in)Tj
1.0374 0 Td
(more)Tj
2.3414 0 Td
(detail.)Tj
2.6419 0 Td
(SBW25)Tj
-32.8873 -1.3039 Td
(RccR)Tj
2.3073 0 Td
(has)Tj
1.5421 0 Td
(a)Tj
0.6576 0 Td
(striking)Tj
3.2938 0 Td
(degree)Tj
2.8517 0 Td
(of)Tj
1.0148 0 Td
(similarity)Tj
4.0081 0 Td
(\(43%)Tj
2.2677 0 Td
(amino)Tj
2.8007 0 Td
(acid)Tj
1.8708 0 Td
(identity,)Tj
3.532 0 Td
(over)Tj
1.9729 0 Td
(70%)Tj
1.9275 0 Td
(similarity\))Tj
4.3483 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
-35.4215 -1.2982 Td
(central)Tj
2.965 0 Td
(metabolic)Tj
4.1442 0 Td
(regulator)Tj
3.8664 0 Td
(HexR.)Tj
2.7213 0 Td
(Examination)Tj
5.3801 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(primary)Tj
3.4583 0 Td
(structure)Tj
3.8211 0 Td
(of)Tj
1.0148 0 Td
(both)Tj
2.0579 0 Td
(proteins)Tj
3.5149 0 Td
(indi-)Tj
-35.4271 -1.2983 Td
(cated)Tj
2.3243 0 Td
(that)Tj
1.7802 0 Td
(the)Tj
1.4683 0 Td
(key)Tj
1.5931 0 Td
(residues)Tj
3.4866 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(DNA)Tj
2.3754 0 Td
(binding)Tj
3.3506 0 Td
(motif)Tj
2.4037 0 Td
(\(R57,)Tj
2.3584 0 Td
(R60\),)Tj
2.3584 0 Td
(and)Tj
1.7292 0 Td
(ligand)Tj
2.7099 0 Td
(binding)Tj
3.3505 0 Td
(site)Tj
-33.7717 -1.2983 Td
(\(S139,)Tj
2.6872 0 Td
(S183\))Tj
2.4604 0 Td
(are)Tj
1.4514 0 Td
(conserved)Tj
4.2576 0 Td
(in)Tj
1.0374 0 Td
(both)Tj
2.058 0 Td
(cases)Tj
2.2223 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8255 0 Td
(1A)Tj
0 g
(\).)Tj
1.9446 0 Td
(To)Tj
1.3379 0 Td
(identify)Tj
3.2939 0 Td
(any)Tj
1.6554 0 Td
(differences)Tj
4.5694 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.4683 0 Td
(two)Tj
-35.7956 -1.3039 Td
(proteins,)Tj
3.736 0 Td
(molecular)Tj
4.2236 0 Td
(models)Tj
3.1011 0 Td
(of)Tj
1.0148 0 Td
(both)Tj
2.0636 0 Td
(were)Tj
2.1203 0 Td
(produced)Tj
4.0478 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2756 0 Td
(four)Tj
1.9105 0 Td
(published)Tj
4.1273 0 Td
(crystal)Tj
2.8119 0 Td
(structures)Tj
-32.9043 -1.2982 Td
(of)Tj
1.0147 0 Td
(transcriptional)Tj
6.1115 0 Td
(regulators)Tj
4.2236 0 Td
(\(PDB)Tj
2.4378 0 Td
(files:)Tj
2.0296 0 Td
(3sho,)Tj
2.3187 0 Td
(2o3f,)Tj
2.1883 0 Td
(4ivn)Tj
1.9673 0 Td
(and)Tj
1.7291 0 Td
(3iwf\).)Tj
2.5001 0 Td
(Both)Tj
2.1373 0 Td
(models)Tj
3.1011 0 Td
(were)Tj
2.126 0 Td
(essen-)Tj
-33.8851 -1.2983 Td
(tially)Tj
2.1656 0 Td
(identical,)Tj
3.8834 0 Td
(with)Tj
1.9956 0 Td
(no)Tj
1.2756 0 Td
(obvious)Tj
3.3505 0 Td
(differences)Tj
4.5638 0 Td
(in)Tj
1.0375 0 Td
(binding)Tj
3.3505 0 Td
(sites)Tj
1.9332 0 Td
(or)Tj
1.0942 0 Td
(residue)Tj
3.1237 0 Td
(conformation)Tj
5.743 0 Td
(emerging)Tj
-33.5166 -1.3039 Td
(from)Tj
2.2053 0 Td
(this)Tj
1.6724 0 Td
(analysis)Tj
3.3279 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1B)Tj
0 g
(\).)Tj
-7.8292 -1.2983 Td
(Despite)Tj
3.2484 0 Td
(their)Tj
2.109 0 Td
(highly)Tj
2.7099 0 Td
(similar)Tj
2.9877 0 Td
(predicted)Tj
3.9968 0 Td
(structures,)Tj
/F8 1 Tf
4.4107 0 Td
[(\r)6()1()]TJ
/F7 1 Tf
2.1713 0 Td
(does)Tj
2.041 0 Td
(not)Tj
1.576 0 Td
(appear)Tj
2.914 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(initial)Tj
2.5512 0 Td
(sugar)Tj
-34.418 -1.2983 Td
(beet)Tj
1.8708 0 Td
(IVET)Tj
2.4378 0 Td
(dataset)Tj
2.9934 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(],)Tj
2.0749 0 Td
(suggesting)Tj
4.405 0 Td
(distinct)Tj
3.2145 0 Td
(regulatory)Tj
4.3143 0 Td
(roles)Tj
2.1316 0 Td
(for)Tj
1.389 0 Td
(RccR)Tj
2.3074 0 Td
(and)Tj
1.7291 0 Td
(HexR.)Tj
2.7269 0 Td
(To)Tj
1.338 0 Td
(test)Tj
1.5987 0 Td
(this,)Tj
1.8992 0 Td
(we)Tj
-36.4306 -1.3039 Td
(examined)Tj
4.1272 0 Td
(the)Tj
1.4626 0 Td
(significance)Tj
4.938 0 Td
(of)Tj
1.0091 0 Td
(both)Tj
/F8 1 Tf
2.0579 0 Td
[()20()7()]TJ
/F7 1 Tf
1.9106 0 Td
(and)Tj
/F8 1 Tf
1.7291 0 Td
[(\r)6()1()]TJ
/F7 1 Tf
2.1713 0 Td
(to)Tj
1.0205 0 Td
(rhizosphere)Tj
4.9322 0 Td
(colonisation)Tj
5.1307 0 Td
(and)Tj
1.7291 0 Td
(plant)Tj
2.2621 0 Td
(interac-)Tj
-34.4804 -1.2982 Td
(tion.)Tj
2.0579 0 Td
(Single)Tj
2.6362 0 Td
(and)Tj
1.7178 0 Td
(double)Tj
/F8 1 Tf
2.948 0 Td
[()20()7()]TJ
/F7 1 Tf
(/)Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
4.1896 0 Td
(mutants)Tj
3.4979 0 Td
(were)Tj
2.1146 0 Td
(produced)Tj
4.0309 0 Td
(in)Tj
/F8 1 Tf
1.0261 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9865 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
4.4106 0 Td
(SBW25,)Tj
3.4073 0 Td
(and)Tj
1.7177 0 Td
(a)Tj
0.6463 0 Td
(series)Tj
-35.3874 -1.2983 Td
(of)Tj
1.0147 0 Td
(competitive)Tj
4.921 0 Td
(colonisation)Tj
5.1306 0 Td
(assays)Tj
2.6249 0 Td
(performed)Tj
4.473 0 Td
(with)Tj
/F8 1 Tf
1.9956 0 Td
[()4( )6(%)]TJ
/F7 1 Tf
(-labelled)Tj
5.3008 0 Td
(WT)Tj
1.7971 0 Td
(SBW25.)Tj
3.4129 0 Td
(After)Tj
2.2904 0 Td
(seven)Tj
2.4264 0 Td
(days,)Tj
-35.3874 -1.3039 Td
(significantly)Tj
5.0853 0 Td
(fewer)Tj
/F14 1 Tf
2.4094 0 Td
( )Tj
/F8 1 Tf
[()20()7()]TJ
/F7 1 Tf
(,)Tj
/F14 1 Tf
2.7269 0 Td
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
2.7609 0 Td
(and)Tj
/F14 1 Tf
1.7292 0 Td
( )Tj
/F8 1 Tf
[()20()7()]TJ
/F14 1 Tf
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
5.0456 0 Td
(colony)Tj
2.9027 0 Td
(forming)Tj
3.4809 0 Td
(units)Tj
2.2223 0 Td
(\(CFUs\))Tj
3.1862 0 Td
(were)Tj
2.1203 0 Td
(recovered)Tj
-33.6697 -1.2983 Td
(from)Tj
2.2053 0 Td
(model)Tj
2.7439 0 Td
(rhizospheres)Tj
5.2894 0 Td
(compared)Tj
4.2463 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4683 0 Td
(WT-)Tj
/F8 1 Tf
[()4( )6(%)]TJ
/F7 1 Tf
3.8211 0 Td
(competitor)Tj
4.6545 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1C)Tj
0 g
(\),)Tj
1.9219 0 Td
(indicating)Tj
4.2689 0 Td
(that)Tj
-34.435 -1.2982 Td
(both)Tj
2.0579 0 Td
(genes)Tj
2.4434 0 Td
(are)Tj
1.4457 0 Td
(similarly)Tj
3.6907 0 Td
(important)Tj
4.2803 0 Td
(for)Tj
1.3889 0 Td
(competitive)Tj
4.9153 0 Td
(growth)Tj
3.0784 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4683 0 Td
(plant)Tj
2.2677 0 Td
(root)Tj
1.9049 0 Td
(environment.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 471.1748 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(HexR)Tj
2.6929 0 Td
(and)Tj
1.8661 0 Td
(RccR)Tj
2.641 0 Td
(are)Tj
1.6441 0 Td
(important)Tj
4.3465 0 Td
(for)Tj
1.3748 0 Td
(bacterial)Tj
3.9449 0 Td
(growth)Tj
3.189 0 Td
(in)Tj
0.9827 0 Td
(different)Tj
3.789 0 Td
(carbon)Tj
-26.471 -1.1669 Td
(sources)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 440.2204 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(We)Tj
1.61 0 Td
(examined)Tj
4.1272 0 Td
(the)Tj
1.4684 0 Td
(responses)Tj
4.0932 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.474 0 Td
[()20()7()]TJ
1.9105 0 Td
[( )16()6()]TJ
1.7291 0 Td
[(\r)6()1()]TJ
/F7 1 Tf
2.177 0 Td
(mutants)Tj
3.5093 0 Td
(to)Tj
1.0261 0 Td
(different)Tj
3.651 0 Td
(nutrient)Tj
3.5036 0 Td
(conditions)Tj
4.4788 0 Td
(by)Tj
-35.773 -1.3039 Td
(testing)Tj
2.8799 0 Td
(their)Tj
2.109 0 Td
(ability)Tj
2.6929 0 Td
(to)Tj
1.0261 0 Td
(grow)Tj
2.2507 0 Td
(in)Tj
1.0318 0 Td
(rich,)Tj
2.0353 0 Td
(complex)Tj
3.6226 0 Td
(and)Tj
1.7292 0 Td
(defined)Tj
3.2258 0 Td
(minimal)Tj
3.6226 0 Td
(media)Tj
2.6929 0 Td
(containing)Tj
4.5298 0 Td
(different)Tj
-33.4486 -1.2982 Td
(sources)Tj
3.1917 0 Td
(of)Tj
1.0148 0 Td
(carbon.)Tj
3.2372 0 Td
(We)Tj
1.6101 0 Td
(observed)Tj
3.7984 0 Td
(little)Tj
1.9955 0 Td
(effect)Tj
2.3641 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()20()7(&\r)6()1()]TJ
/F7 1 Tf
4.1782 0 Td
(gene)Tj
2.0807 0 Td
(deletion)Tj
3.4639 0 Td
(in)Tj
1.0375 0 Td
(rich)Tj
1.8084 0 Td
(media;)Tj
2.914 0 Td
(no)Tj
-33.7093 -1.2983 Td
(growth-rate)Tj
4.9492 0 Td
(difference)Tj
4.2066 0 Td
(in)Tj
1.0318 0 Td
(KB,)Tj
1.7008 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.6576 0 Td
(slight)Tj
2.3868 0 Td
(delay)Tj
2.3017 0 Td
(in)Tj
1.0375 0 Td
(log)Tj
1.44 0 Td
(phase)Tj
2.4831 0 Td
(entry)Tj
2.3131 0 Td
(for)Tj
1.3889 0 Td
(both)Tj
2.058 0 Td
(mutants)Tj
3.5092 0 Td
(in)Tj
1.0375 0 Td
(LB)Tj
-34.2309 -1.3039 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(2A/2B)Tj
0 g
(\).)Tj
3.3278 0 Td
(Conversely,)Tj
4.8983 0 Td
(deletion)Tj
3.4639 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()20()7()]TJ
/F7 1 Tf
1.9162 0 Td
(resulted)Tj
3.3959 0 Td
(in)Tj
1.0374 0 Td
(severely)Tj
3.3789 0 Td
(compromised)Tj
5.7657 0 Td
(bacterial)Tj
3.6226 0 Td
(growth)Tj
3.0727 0 Td
(in)Tj
-36.714 -1.2983 Td
(minimal)Tj
3.6226 0 Td
(medium)Tj
3.6057 0 Td
(with)Tj
1.9955 0 Td
(either)Tj
2.5342 0 Td
(glucose)Tj
3.1691 0 Td
(or)Tj
1.0998 0 Td
(glycerol)Tj
3.3562 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(sole)Tj
1.7575 0 Td
(carbon)Tj
3.0104 0 Td
(source)Tj
2.8346 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(2C/2D)Tj
0 g
(\).)Tj
3.464 0 Td
(Dele-)Tj
-34.7582 -1.2982 Td
(tion)Tj
1.8425 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[(\r)6()1()]TJ
/F7 1 Tf
2.1713 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(other)Tj
2.3471 0 Td
(hand)Tj
2.262 0 Td
(affected)Tj
3.3222 0 Td
(growth)Tj
3.0784 0 Td
(in)Tj
1.0318 0 Td
(media)Tj
2.6929 0 Td
(containing)Tj
4.5297 0 Td
(pyruvate,)Tj
3.9288 0 Td
(acetate)Tj
2.9537 0 Td
(or)Tj
1.0942 0 Td
(succi-)Tj
-35.0133 -1.304 Td
(nate,)Tj
2.1486 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(2E,)Tj
1.491 0 Td
(2F)Tj
1.2246 0 Td
(and)Tj
1.7291 0 Td
(2G)Tj
0 g
(\).)Tj
1.9786 0 Td
(These)Tj
2.5625 0 Td
(growth)Tj
3.0727 0 Td
(defects)Tj
2.9707 0 Td
(were)Tj
2.1203 0 Td
(dominant)Tj
4.1726 0 Td
(and)Tj
1.7291 0 Td
(not)Tj
1.5704 0 Td
(additive:)Tj
3.651 0 Td
(the)Tj
/F14 1 Tf
1.4683 0 Td
( )Tj
/F8 1 Tf
[()20()7(&)]TJ
/F14 1 Tf
-33.7093 -1.2982 Td
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
2.7609 0 Td
(double)Tj
2.9593 0 Td
(mutant)Tj
3.1465 0 Td
(displayed)Tj
3.9798 0 Td
(an)Tj
/F8 1 Tf
1.2019 0 Td
[()20()7()]TJ
/F7 1 Tf
(-mutant)Tj
5.193 0 Td
(phenotype)Tj
4.439 0 Td
(in)Tj
1.0375 0 Td
(glycerol)Tj
3.3562 0 Td
(and)Tj
1.7291 0 Td
(glucose,)Tj
3.4016 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
/F8 1 Tf
-34.9339 -1.2983 Td
[(\r)6()1()24()]TJ
/F7 1 Tf
(mutant)Tj
5.4311 0 Td
(phenotype)Tj
4.439 0 Td
(in)Tj
1.0318 0 Td
(pyruvate,)Tj
3.9288 0 Td
(acetate,)Tj
3.1805 0 Td
(or)Tj
1.0998 0 Td
(succinate)Tj
3.9231 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8199 0 Td
(2)Tj
0 g
(\).)Tj
1.2642 0 Td
(Interestingly,)Tj
5.4935 0 Td
(an)Tj
1.2019 0 Td
(exception)Tj
4.0932 0 Td
(is)Tj
-36.9068 -1.2983 Td
(the)Tj
1.4683 0 Td
(bacteria)Tj
3.3789 0 Td
(growth)Tj
3.0727 0 Td
(in)Tj
1.0375 0 Td
(acetate,)Tj
3.1804 0 Td
(in)Tj
1.0318 0 Td
(which)Tj
2.6532 0 Td
(the)Tj
1.4684 0 Td
(double)Tj
2.9593 0 Td
(mutant)Tj
3.1465 0 Td
(shows)Tj
2.6588 0 Td
(an)Tj
1.2076 0 Td
(intermediate)Tj
5.3178 0 Td
(ability)Tj
2.6985 0 Td
(to)Tj
-35.2797 -1.3039 Td
(grow)Tj
2.245 0 Td
(compared)Tj
4.2462 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(wild)Tj
1.9446 0 Td
(type)Tj
1.9162 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(single)Tj
2.5342 0 Td
(mutants)Tj
3.5036 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(2F)Tj
0 g
(\).)Tj
1.7915 0 Td
(Despite)Tj
3.2485 0 Td
(their)Tj
2.1033 0 Td
(marked)Tj
-31.0448 -1.2982 Td
(sequence)Tj
3.855 0 Td
(and)Tj
1.7348 0 Td
(structural)Tj
4.0762 0 Td
(similarities,)Tj
4.8302 0 Td
(the)Tj
1.4684 0 Td
(activities)Tj
3.7133 0 Td
(of)Tj
1.0148 0 Td
(RccR/HexR)Tj
4.9209 0 Td
(are)Tj
1.4457 0 Td
(clearly)Tj
2.8233 0 Td
(distinct,)Tj
3.4356 0 Td
(with)Tj
1.9955 0 Td
(their)Tj
-35.3137 -1.2983 Td
(relative)Tj
3.135 0 Td
(importance)Tj
4.8303 0 Td
(varying)Tj
3.2201 0 Td
(markedly)Tj
3.9911 0 Td
(depending)Tj
4.4674 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.474 0 Td
(available)Tj
3.6623 0 Td
(carbon)Tj
3.0161 0 Td
(sources.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F6 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(P)Tj
/F0 1 Tf
(.)Tj
/F6 1 Tf
1.1574 0 Td
(fluorescens)Tj
/F0 1 Tf
5.2725 0 Td
(HexR)Tj
2.6977 0 Td
(is)Tj
0.9307 0 Td
(a)Tj
0.7654 0 Td
(regulator)Tj
4.115 0 Td
(of)Tj
1.0488 0 Td
(glucose)Tj
3.6189 0 Td
(metabolism)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Previous)Tj
3.6793 0 Td
(studies)Tj
2.982 0 Td
(have)Tj
2.0523 0 Td
(shown)Tj
2.8403 0 Td
(that)Tj
1.7688 0 Td
(HexR)Tj
2.4888 0 Td
(is)Tj
0.8391 0 Td
(a)Tj
0.6462 0 Td
(transcriptional)Tj
6.1058 0 Td
(repressor)Tj
3.9175 0 Td
(of)Tj
1.0035 0 Td
(the)Tj
1.4626 0 Td
(glucose)Tj
3.1691 0 Td
(phosphory-)Tj
-32.9553 -1.2982 Td
(lative)Tj
2.3414 0 Td
(and)Tj
1.7291 0 Td
(Entner-Doudoroff)Tj
7.5911 0 Td
(pathways)Tj
3.9345 0 Td
(in)Tj
/F8 1 Tf
1.0318 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9978 0 Td
[()11()14(')7( )4()4( )]TJ
/F7 1 Tf
2.7666 0 Td
(and)Tj
/F8 1 Tf
1.7348 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[( )6()2()-9()10()-8( )12()6()7()-3( )]TJ
/F7 1 Tf
4.4787 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(31)Tj
0 g
(].)Tj
1.7348 0 Td
(HexR)Tj
2.5001 0 Td
(represses)Tj
-33.5676 -1.2983 Td
(the)Tj
1.4683 0 Td
(expression)Tj
4.4674 0 Td
(of)Tj
1.0148 0 Td
(its)Tj
1.1451 0 Td
(gene)Tj
2.0807 0 Td
(targets)Tj
2.8686 0 Td
(by)Tj
1.1849 0 Td
(binding)Tj
3.3505 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(specific)Tj
3.1918 0 Td
(consensus)Tj
4.2803 0 Td
(sequence)Tj
3.8607 0 Td
(5'-)Tj
1.2586 0 Td
(TTGT-N)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 563.0173 198.3118 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(7-)Tj
-48.4012 -1.9476 Td
(8)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 203.5842 186.9732 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(-ACAA-3')Tj
4.3256 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(3A)Tj
0 g
(\).)Tj
1.9446 0 Td
(To)Tj
1.3379 0 Td
(verify)Tj
2.4775 0 Td
(the)Tj
1.474 0 Td
(HexR)Tj
2.4945 0 Td
(regulatory)Tj
4.3199 0 Td
(system)Tj
2.9367 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(3B)Tj
0 g
(\))Tj
1.6158 0 Td
(in)Tj
/F8 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()-36()12()4()11()20()8()-3()7()2()]TJ
/F7 1 Tf
(,)Tj
4.6488 0 Td
(we)Tj
1.3266 0 Td
(first)Tj
-34.9282 -1.2982 Td
(examined)Tj
4.1271 0 Td
(the)Tj
1.4683 0 Td
(intergenic)Tj
4.2577 0 Td
(regions)Tj
3.1634 0 Td
(between)Tj
3.5319 0 Td
(the)Tj
1.4684 0 Td
(p-)Tj
/F8 1 Tf
[()7()4()]TJ
/F7 1 Tf
2.4718 0 Td
(and)Tj
1.7291 0 Td
(p-)Tj
/F8 1 Tf
[()2( )12()]TJ
/F7 1 Tf
(,)Tj
3.4866 0 Td
(and)Tj
1.7291 0 Td
(between)Tj
3.5263 0 Td
(the)Tj
1.4683 0 Td
(p-)Tj
/F8 1 Tf
[(\n)-24()]TJ
/F7 1 Tf
2.4321 0 Td
(and)Tj
1.7292 0 Td
(p-)Tj
/F8 1 Tf
-36.5893 -1.2983 Td
[(\r)6()1()]TJ
/F7 1 Tf
2.1712 0 Td
(promoters)Tj
4.3994 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(SBW25)Tj
3.1918 0 Td
(genome)Tj
3.4072 0 Td
(for)Tj
1.3833 0 Td
(the)Tj
1.474 0 Td
(HexR)Tj
2.4945 0 Td
(consensus)Tj
4.2802 0 Td
(binding)Tj
3.3562 0 Td
(sequence.)Tj
4.0819 0 Td
(As)Tj
-32.7455 -1.2982 Td
(expected,)Tj
3.9457 0 Td
(both)Tj
2.0636 0 Td
(intergenic)Tj
4.2519 0 Td
(regions)Tj
3.1635 0 Td
(contained)Tj
4.2009 0 Td
(HexR)Tj
2.4945 0 Td
(binding)Tj
3.3562 0 Td
(sites.)Tj
2.1543 0 Td
(Next,)Tj
2.3697 0 Td
(qRT-PCR)Tj
4.1499 0 Td
(experiments)Tj
-32.1502 -1.304 Td
(were)Tj
2.1202 0 Td
(performed)Tj
4.473 0 Td
(to)Tj
1.0205 0 Td
(examine)Tj
3.6056 0 Td
(the)Tj
1.4684 0 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.474 0 Td
[()2( )12()]TJ
/F7 1 Tf
(,)Tj
/F8 1 Tf
2.6135 0 Td
[()7()4()]TJ
/F7 1 Tf
1.6044 0 Td
(and)Tj
/F8 1 Tf
1.7291 0 Td
[(\n)-24()]TJ
/F7 1 Tf
1.5648 0 Td
(genes)Tj
2.4434 0 Td
(in)Tj
1.0318 0 Td
(WT)Tj
1.7972 0 Td
(and)Tj
/F14 1 Tf
1.7348 0 Td
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
-34.1572 -1.2982 Td
(backgrounds,)Tj
5.6068 0 Td
(for)Tj
1.3776 0 Td
(bacteria)Tj
3.3732 0 Td
(grown)Tj
2.7893 0 Td
(in)Tj
1.0261 0 Td
(minimal)Tj
3.617 0 Td
(media)Tj
2.6816 0 Td
(containing)Tj
4.5184 0 Td
(different,)Tj
3.8777 0 Td
(single)Tj
2.5229 0 Td
(carbon)Tj
3.0047 0 Td
(sources.)Tj
-34.3953 -1.2983 Td
(While)Tj
2.6474 0 Td
(little)Tj
2.0013 0 Td
(difference)Tj
4.2066 0 Td
(in)Tj
1.0318 0 Td
(expression)Tj
4.4673 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2564 0 Td
(genes)Tj
2.4378 0 Td
(was)Tj
1.7008 0 Td
(observed)Tj
3.7927 0 Td
(between)Tj
3.5319 0 Td
(WT)Tj
1.7972 0 Td
(and)Tj
/F14 1 Tf
1.7291 0 Td
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
2.7666 0 Td
(for)Tj
-35.3817 -1.3039 Td
(bacteria)Tj
3.3788 0 Td
(grown)Tj
2.7949 0 Td
(in)Tj
1.0375 0 Td
(glucose)Tj
3.1691 0 Td
(minimal)Tj
3.6226 0 Td
(media)Tj
2.6929 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8199 0 Td
(3C)Tj
0 g
(\),)Tj
1.9275 0 Td
(the)Tj
1.4684 0 Td
(expression)Tj
4.4673 0 Td
(profile)Tj
2.8403 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F15 1 Tf
1.4684 0 Td
( )Tj
/F8 1 Tf
[(\r)6()1()]TJ
/F7 1 Tf
2.7666 0 Td
(mutant)Tj
-34.469 -1.2983 Td
(significantly)Tj
5.0852 0 Td
(diverged)Tj
3.685 0 Td
(from)Tj
2.2054 0 Td
(WT)Tj
1.7971 0 Td
(when)Tj
2.4038 0 Td
(the)Tj
1.4683 0 Td
(strains)Tj
2.863 0 Td
(were)Tj
2.1203 0 Td
(grown)Tj
2.7949 0 Td
(in)Tj
1.0375 0 Td
(glycerol,)Tj
3.583 0 Td
(pyruvate)Tj
3.702 0 Td
(or)Tj
1.0998 0 Td
(acetate)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 133.6819 23.6976 re
W* n
q
133.6252 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 396.7937 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Transcrip)Tj
4.096 0 Td
(tional)Tj
2.5583 0 Td
(control)Tj
3.1607 0 Td
(of)Tj
/F6 1 Tf
1.0417 0 Td
(Pseudomo)Tj
4.7056 0 Td
(nas)Tj
/F0 1 Tf
1.8071 0 Td
(metabolism)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
95.5276 34.9228 m
251.8299 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(Genetics)Tj
4.1102 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4749 0 Td
(https://)Tj
2.9551 0 Td
(doi.org/10.13)Tj
5.7331 0 Td
(71/journal.p)Tj
5.1237 0 Td
(gen.100)Tj
3.5433 0 Td
(6839)Tj
0 g
3.1819 0 Td
(June)Tj
2.3528 0 Td
(28,)Tj
1.5803 0 Td
(2017)Tj
33.2294 0 Td
(4)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
118 0 obj
<>stream
endstream
endobj
119 0 obj
<>stream
endstream
endobj
120 0 obj
<>stream
endstream
endobj
121 0 obj
<>stream
endstream
endobj
122 0 obj
<>stream
endstream
endobj
123 0 obj
<>stream
endstream
endobj
124 0 obj
<>stream
endstream
endobj
125 0 obj
<>stream
BT
66.402 0 Td
(29)Tj
ET
Q
endstream
endobj
126 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 13 0 R/Annots 128 0 R/Contents 129 0 R/TrimBox[0 0 612 792]>>
endobj
127 0 obj
<>stream
x\QD AFEAV ^[XQ۵[;gayy;=w{s?"@ D"@ D"@ D"@ D"@ D"@ D"@ D"@ D"@ D"@+>|ɓ?~>j D"@ D"@ )VպU+ךݞSQ&"@ D"@ D" ҥKYZ[ZZ~Jt`&R# D"@ D"@@ X?LL<