The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio, Desirè Di, Maccarini, Marco, Parker, Roger, Mackie, Alan, Fragneto, Giovanna and Bombelli, Francesca Baldelli (2017) The effect of the protein corona on the interaction between nanoparticles and lipid bilayers. Journal of Colloid and Interface Science, 504. 741–750. ISSN 0021-9797

[thumbnail of JCIS_2017]
PDF (JCIS_2017) - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB) | Preview


Hypothesis: It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. Experiments: In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. Findings: Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.

Item Type: Article
Uncontrolled Keywords: supported lipid bilayer,protein corona nanoparticles,quartz crystal microbalance,neutron reflectometry,soft corona,hard corona
Faculty \ School: Faculty of Science > School of Pharmacy
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: Pure Connector
Date Deposited: 03 Jun 2017 05:08
Last Modified: 22 Oct 2022 02:43
DOI: 10.1016/j.jcis.2017.05.086

Actions (login required)

View Item View Item