Enhanced biodegradation of PAHs in historically contaminated soil by M. gilvum inoculated biochar

Xiong, Bijing, Zhang, Youchi, Hou, Yanwei, Arp, Hans Peter H., Reid, Brian J. ORCID: https://orcid.org/0000-0002-9613-979X and Cai, Chao (2017) Enhanced biodegradation of PAHs in historically contaminated soil by M. gilvum inoculated biochar. Chemosphere, 182. 316–324. ISSN 0045-6535

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The inoculation of rice straw biochar with PAH-degrading Mycobacterium gilvum (1.27 × 1011 ± 1.24 × 1010 cell g−1), and the subsequent amendment of this composite material to PAHs contaminated (677 mg kg−1) coke plant soil, was conducted in order to investigate if would enhance PAHs biodegradation in soils. The microbe-biochar composite showed superior degradation capacity for phenanthrene, fluoranthene and pyrene. Phenanthrene loss in the microbe-biochar composite, free cell alone and biochar alone treatments was, respectively, 62.6 ± 3.2%, 47.3 ± 4.1% and non-significant (P > 0.05); whereas for fluoranthene loss it was 52.1 ± 2.3%; non-significant (P > 0.05) and non-significant (P > 0.05); and for pyrene loss it was 62.1 ± 0.9%; 19.7 ± 6.5% and 13.5 ± 2.8%. It was hypothesized that the improved remediation was underpinned by i) biochar enhanced mass transfer of PAHs from the soil to the carbonaceous biochar “sink”, and ii) the subsequent degradation of the PAHs by the immobilized M. gilvum. To test this mechanism, a surfactant (Brij 30; 20 mg g−1 soil), was added to impede PAHs mass transfer to biochar and sorption. The surfactant increased solution phase PAH concentrations and significantly (P < 0.05) reduced PAH degradation in the biochar immobilized M. gilvum treatments; indicating the enhanced degradation occurred between the immobilized M. gilvum and biochar sorbed PAHs.

Item Type: Article
Uncontrolled Keywords: pahs,biochar,microbe immobilization,soil,degradation
Faculty \ School: Faculty of Science > School of Environmental Sciences
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Environmental Biology
Faculty of Science > Research Groups > Resources, Sustainability and Governance (former - to 2018)
Faculty of Science > Research Groups > Geosciences
Faculty of Science > Research Groups > Geosciences and Natural Hazards (former - to 2017)
Depositing User: Pure Connector
Date Deposited: 09 May 2017 05:08
Last Modified: 22 Oct 2022 02:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/63404
DOI: 10.1016/j.chemosphere.2017.05.020


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