Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis

Yu, Guotai, Champouret, Nicolas, Steuernagel, Burkhard, Olivera, Pablo D., Simmons, Jamie, Williams, Cole, Johnson, Ryan, Moscou, Matthew J., Hernández-Pinzón, Inmaculada, Green, Phon, Sela, Hanan, Millet, Eitan, Jones, Jonathan D. G., Ward, Eric R., Steffenson, Brian J. and Wulff, Brande B. H. (2017) Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis. Theoretical and Applied Genetics, 130 (6). 1207–1222. ISSN 0040-5752

[img]
Preview
PDF (Published manuscript) - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview

Abstract

Stem rust is one of the most important diseases of wheat in the world. When single stem rust resistance (Sr) genes are deployed in wheat, they are often rapidly overcome by the pathogen. To this end, we initiated a search for novel sources of resistance in diverse wheat relatives and identified the wild goat grass species Aegilops sharonesis (Sharon goatgrass) as a substantial reservoir of resistance to wheat stem rust. The objectives of this study were to discover and map novel Sr genes in Ae. sharonensis and to explore the possibility of identifying new Sr genes by genome-wide association study (GWAS). We developed two biparental populations between resistant and susceptible accessions of Ae. sharonensis and performed QTL and linkage analysis. In an F6 recombinant inbred line and an F2 population, two genes were identified that mapped to the short arm of chromosome 1Ssh, designated as Sr-1644-1Sh, and the long arm of chromosome 5Ssh, designated as Sr-1644-5Sh. The gene Sr-1644-1Sh confers a high level of resistance to race TTKSK (one of the Ug99 lineage races), while the gene Sr-1644-5Sh conditions strong resistance to TRTTF, another widely virulent race found in Yemen. Additionally, GWAS was conducted on 125 diverse Ae. sharonensis accessions for stem rust resistance. The gene Sr-1644-1Sh was detected by GWAS, while Sr-1644-5Sh was not detected, indicating that the effectiveness of GWAS might be affected by marker density, population structure, low allele frequency and other factors.

Item Type: Article
Faculty \ School:

Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 15 Mar 2017 01:40
Last Modified: 27 Jul 2020 23:55
URI: https://ueaeprints.uea.ac.uk/id/eprint/62957
DOI: 10.1007/s00122-017-2882-8

Actions (login required)

View Item View Item