High performance reduction of H2O2 with an electron transport decaheme cytochrome on a porous ITO electrode

Reuillard, Bertrand, H. Ly, Khoa, Hildebrandt, Peter, Jeuken, Lars J. C., Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 and Reisner, Erwin (2017) High performance reduction of H2O2 with an electron transport decaheme cytochrome on a porous ITO electrode. Journal of the American Chemical Society, 139 (9). 3324–3327. ISSN 0002-7863

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Abstract

A decaheme cytochrome MtrC from Shewanella oneidensis MR-1 immobilized on an ITO electrode displays unprecedented H2O2 reduction activity. Although MtrC showed lower peroxidase activity in solution compared to horseradish peroxidase, the ten heme cofactors enable excellent electronic communication and a superior activity on the electrode surface. A hierarchical ITO electrode enabled optimal immobilization of MtrC and a high current density of 1 mA cm−2 at 0.4 V vs SHE could be obtained at pH 6.5 (Eonset = 0.72 V). UV−visible and Resonance Raman spectroelectrochemical studies suggest the formation of a high valent iron-oxo species as the catalytic intermediate. Our findings demonstrate the potential of heme-proteins to catalyze technologically relevant reactions and establish MtrC as a new benchmark in biotechnological H2O2 reduction with scope for applications in fuel cells and biosensors.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory
Depositing User:	Pure Connector
Date Deposited:	04 Mar 2017 01:41
Last Modified:	22 Oct 2022 02:20
URI:	https://ueaeprints.uea.ac.uk/id/eprint/62850
DOI:	10.1021/jacs.6b12437

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