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Omidvar, Vahid, Mohorianu, Irina, Dalmay, Tamas 
ORCID: https://orcid.org/0000-0003-1492-5429, Zheng, Yi, Fei, Zhangjun, Pucci, Anna, Mazzucato, Andrea, Večeřová, Vendula, Sedlářova, Michaela and Fellner, Martin
(2017)
Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant.
PLoS One, 12 (2).
ISSN 1932-6203
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Abstract
The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 7B-1, transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > School of Computing Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 01 Mar 2017 01:46 |
| Last Modified: | 22 Oct 2022 02:23 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/62800 |
| DOI: | 10.1371/journal.pone.0170715 |
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