Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy

Penon, Oriol, Marín, María J., Russell, David A. and Pérez-García, Lluïsa (2017) Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy. Journal of Colloid and Interface Science, 496. 100–110. ISSN 0021-9797

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Abstract

Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitisers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitisers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognises the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesised following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterised using UV-Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesised using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalised with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells.

Item Type: Article
Uncontrolled Keywords: photodynamic therapy,multifunctional nanoparticles,porphyrins,gold nanoparticles (aunps),water soluble nanoconjugates,targeting antibodies,singlet oxygen production,in vitro phototoxicity,human breast cancer cells
Faculty \ School: Faculty of Science > School of Chemistry
Depositing User: Pure Connector
Date Deposited: 16 Feb 2017 02:24
Last Modified: 01 Nov 2019 01:05
URI: https://ueaeprints.uea.ac.uk/id/eprint/62626
DOI: 10.1016/j.jcis.2017.02.006

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