Diagnosing tuberculosis in the 21st century – Dawn of a genomics revolution?

Jeanes, Christopher and O'Grady, Justin (2016) Diagnosing tuberculosis in the 21st century – Dawn of a genomics revolution? International Journal of Mycobacteriology, 5 (4). pp. 384-391. ISSN 2212-5531

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Abstract

Tuberculosis (TB) ranks alongside HIV as the leading cause of death worldwide, killing 1.5 million people in 2014. Traditional laboratory techniques do not provide sufficiently rapid results to inform clinicians on appropriate treatment, especially in the face of increasingly prevalent drug-resistant TB. Rapid molecular methods such as PCR and LAMP are vital tools in the fight against TB, however, rapid advances in next generation sequencing (NGS) technology are allowing increasingly rapid and accurate sequencing of entire bacterial genomes at ever decreasing cost, providing unprecedented depth of information. These advances mean NGS stands to revolutionise the diagnosis and epidemiological study of Mycobacterium tuberculosis infection. This review focuses on current applications of NGS for TB diagnosis including sequencing cultured isolates to predict drug resistance and, more desirably, direct diagnostic metagenomic sequencing of clinical samples. Also discussed is the potential impact of NGS on the epidemiological study of TB and some of the key challenges that need to be overcome to enable this promising technology to be translated into routine use.

Item Type: Article
Additional Information: Copyright 2016 Asian-African Society for Mycobacteriology. Production and hosting by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Uncontrolled Keywords: tuberculosis,diagnostics,whole genome sequencing,next generation sequencing,genomics
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 14 Dec 2016 00:07
Last Modified: 22 Jul 2020 01:16
URI: https://ueaeprints.uea.ac.uk/id/eprint/61709
DOI: 10.1016/j.ijmyco.2016.11.028

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