Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels:Mitochondrial carriers migrate as monomers not dimers

Crichton, Paul G., Harding, Marilyn, Ruprecht, Jonathan J., Lee, Yang and Kunji, Edmund R. S. (2013) Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels:Mitochondrial carriers migrate as monomers not dimers. Journal of Biological Chemistry, 288 (30). pp. 22163-22173. ISSN 0021-9258

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Abstract

Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels.  Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain.  Conclusion: The mitochondrial carriers are monomeric not dimeric.  Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction.

Item Type: Article
Additional Information: Creative Commons: CC-BY license
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Pure Connector
Date Deposited: 03 Nov 2016 12:00
Last Modified: 15 Nov 2020 00:46
URI: https://ueaeprints.uea.ac.uk/id/eprint/61227
DOI: 10.1074/jbc.M113.484329

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