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Crichton, Paul G. 
ORCID: https://orcid.org/0000-0003-3786-8359, Harding, Marilyn, Ruprecht, Jonathan J., Lee, Yang and Kunji, Edmund R. S.
(2013)
Lipid, detergent, and coomassie blue G-250 affect the migration of small membrane proteins in blue native gels:Mitochondrial carriers migrate as monomers not dimers.
Journal of Biological Chemistry, 288 (30).
pp. 22163-22173.
ISSN 0021-9258
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Abstract
Background: Mitochondrial carriers were thought to be dimeric based on their migration in blue native gels. Results: The high molecular mass species observed in blue native gels are composed of protein monomers, detergent, lipid, and Coomassie stain. Conclusion: The mitochondrial carriers are monomeric not dimeric. Significance: The apparent mass of small membrane proteins in blue native gels requires significant correction.
| Item Type: | Article |
|---|---|
| Additional Information: | Creative Commons: CC-BY license |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 03 Nov 2016 12:00 |
| Last Modified: | 19 Oct 2023 01:51 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/61227 |
| DOI: | 10.1074/jbc.M113.484329 |
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